1st microbio lecture - Essence, History, Taxonomy 2 - converted

Created by Harris

p.9

Joseph Lister introduced aseptic techniques that involved disinfection of hands using ___ prior to surgery.

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p.9

chemicals

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p.9
Aseptic Techniques and Sterilization

Joseph Lister introduced aseptic techniques that involved disinfection of hands using ___ prior to surgery.

chemicals

p.9
Aseptic Techniques and Sterilization

Joseph Lister's techniques also included the use of ___ for sterilization.

heat

p.10
Germ Theory of Disease

Many diseases are caused by the growth of ___ in the body and not by sins, bad character, or poverty, etc.

microbes

p.10
Key Figures in Microbiology

The two major contributors to the germ theory of disease are ___ and ___.

Louis Pasteur, Robert Koch

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Key Figures in Microbiology

Louis Pasteur showed that ___ caused fermentation and spoilage.

microbes

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Key Figures in Microbiology

Louis Pasteur disproved the theory of ___ generation of microorganisms.

spontaneous

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Key Figures in Microbiology

Louis Pasteur developed a process known as ___.

pasteurization

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Key Figures in Microbiology

Louis Pasteur demonstrated what is now known as the ___ Theory of Disease.

Germ

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Key Figures in Microbiology

Louis Pasteur developed a vaccine for ___.

rabies

p.12
Key Figures in Microbiology

Robert Koch established ___'s postulates, a sequence of experimental steps that verified the germ theory.

Koch

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Infectious Diseases and Their Impact

Robert Koch identified the cause of ___, TB, and cholera.

anthrax

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Modern Techniques in Microbiology

Robert Koch developed ___ culture methods.

pure

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Key Figures in Microbiology

Robert Koch was awarded the Nobel Prize in ___ for his contributions to microbiology.

1905

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Vaccination and Chemotherapy Advances

___ is known for the smallpox vaccination developed in 1796.

Jenner

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Vaccination and Chemotherapy Advances

About ___ years after Jenner, Pasteur demonstrated how vaccinations work.

100

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Vaccination and Chemotherapy Advances

Pasteur's work involved the creation of ___ strains of bacteria during extended laboratory cultivation.

avirulent

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Vaccination and Chemotherapy Advances

In ___, Paul Ehrlich developed a synthetic arsenic drug called salvarsan to treat syphilis.

1910

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Vaccination and Chemotherapy Advances

The synthesis of ___ began in the 1930s.

sulfonamides

p.14
Vaccination and Chemotherapy Advances

In ___, Alexander Fleming discovered the first antibiotic, Penicillin.

1928

p.15
Modern Techniques in Microbiology

___, ___, ___, ___, and ___ are the main branches of microbiology.

Bacteriology, Mycology, Parasitology, Virology, Immunology

p.15
Modern Techniques in Microbiology

Microbial genetics and molecular biology have led to ___ technology.

Recombinant DNA

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Modern Techniques in Microbiology

The prokaryotic model system used in recombinant DNA technology is ___ .

E. coli

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Key Figures in Microbiology

In 1901, ___ was awarded the Nobel Prize for the development of Diphtheria antitoxin.

von Behring

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Key Figures in Microbiology

The Nobel Prize in 1902 was awarded to ___ for his work on malaria transmission.

Ross

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Key Figures in Microbiology

___ discovered the TB bacterium and received the Nobel Prize in 1905.

Koch

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Key Figures in Microbiology

The Nobel Prize in 1908 was awarded to ___ for his research on phagocytes.

Metchnikoff

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Vaccination and Chemotherapy Advances

In 1945, the Nobel Prize was awarded to Fleming, Chain, and Florey for their discovery of ___.

Penicillin

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Vaccination and Chemotherapy Advances

___ was awarded the Nobel Prize in 1952 for the discovery of Streptomycin.

Waksman

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Key Figures in Microbiology

The Nobel Prize in 1969 was awarded to Delbrück, Hershey, and Luria for their work on ___.

Viral replication

p.16
Key Figures in Microbiology

In 1987, ___ received the Nobel Prize for his research on antibody genetics.

Tonegawa

p.16
Key Figures in Microbiology

___ was awarded the Nobel Prize in 1997 for his work on prions.

Prusiner

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Infectious Diseases and Their Impact

In 2005, Marshall and Warren received the Nobel Prize for their discovery of ___ and its relation to ulcers.

H. pylori

p.16
Infectious Diseases and Their Impact

The Nobel Prize in 2008 was awarded to Harald zur Hausen for his research on ___ and cervical cancer.

HPV

p.16
Infectious Diseases and Their Impact

Françoise Barré-Sinoussi and Luc Montagnier were awarded the Nobel Prize for their discovery of ___.

HIV

p.17
Microbial Taxonomy and Classification

The formal system of taxonomy was originated by ___ ___ ___.

Carl von Linné

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Microbial Taxonomy and Classification

Taxonomy is concerned with ___, which is the orderly arrangement of organisms into groups.

classification

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Microbial Taxonomy and Classification

In taxonomy, ___ refers to assigning names to organisms.

nomenclature

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Microbial Taxonomy and Classification

The process of discovering and recording traits of organisms for placement into taxonomic schemes is known as ___.

identification

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Microbial Taxonomy and Classification

Living organisms are classified into three domains: ___, ___, and ___.

Bacteria, Archaea, Eukarya

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Microbial Taxonomy and Classification

The Domain Eukarya includes the kingdoms ___, ___, and ___.

Fungi, Plantae, Animalia

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Microbial Taxonomy and Classification

All organisms evolved from cells that formed over ___ billion years ago.

3

p.18
Microbial Taxonomy and Classification

The DNA passed on from ancestors is described as ___.

conserved

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Microbial Taxonomy and Classification

The three domains of life include Bacteria, ___, and Eukarya.

Archaea

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Microbial Taxonomy and Classification

___ are true bacteria characterized by peptidoglycan.

Bacteria

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Microbial Taxonomy and Classification

Archaea are known for living in ___ environments.

extreme

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Microbial Taxonomy and Classification

Eukarya are distinguished by having a ___ and organelles.

nucleus

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Microbial Taxonomy and Classification

Morphological characteristics are used for ___ but not for classification.

identification

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Microbial Taxonomy and Classification

Differential staining is used for ___ and ___ in bacteria.

classification, identification

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Microbial Taxonomy and Classification

Biochemical testing is used for ___ only.

identification

p.20
Microbial Taxonomy and Classification

Serology is used for ___ only.

identification

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Microbial Taxonomy and Classification

Phage typing is used for ___ only.

identification

p.20
Microbial Taxonomy and Classification

Fatty acid profiles are used for ___ only.

identification

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Microbial Taxonomy and Classification

Flow cytometry is used for ___ only.

identification

p.20
Microbial Taxonomy and Classification

DNA base composition is used for ___ but not for identification.

classification

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Microbial Taxonomy and Classification

DNA fingerprinting is used for both ___ and ___.

classification, identification

p.20
Microbial Taxonomy and Classification

PCR is used for both ___ and ___.

classification, identification

p.20
Microbial Taxonomy and Classification

Nucleic acid hybridization techniques are used for both ___ and ___.

classification, identification

p.20
Microbial Taxonomy and Classification

rRNA sequencing is used for ___ but not for identification.

classification

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Microbial Taxonomy and Classification

Morphological characteristics are useful for identifying ___.

eukaryotes

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Microbial Taxonomy and Classification

The process of ___ staining is used in differential staining.

Gram

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Microbial Taxonomy and Classification

Biochemical tests determine the presence of ___ enzymes.

bacterial

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Microbial Taxonomy and Classification

In numerical rapid identification, the ID value is determined by adding the numbers from each group of ___.

tests

p.21
Microbial Taxonomy and Classification

The organism identified with an ID value of 21006 is ___.

Proteus mirabilis

p.22
Importance of Microorganisms

Serology involves reactions of microorganisms with specific antibodies: ___ known anti-serum with unknown bacterium.

Combine

p.22
Importance of Microorganisms

Serology is useful in determining the identity of strains and species, as well as ___ among organisms.

relationships

p.23
Microbial Taxonomy and Classification

Phage typing is used for the identification of bacterial species and strains by determining their susceptibility to various ___.

phages

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Microbial Taxonomy and Classification

DNA fingerprinting uses the number and sizes of DNA fragments produced by ___ digests to determine genetic similarities.

RE

p.24
Microbial Taxonomy and Classification

___ sequencing is a method used in ribotyping.

rRNA

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Microbial Taxonomy and Classification

The ___ can be used to amplify a small amount of microbial DNA in a sample.

Polymerase chain reaction (PCR)

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Microbial Taxonomy and Classification

The presence or identification of an organism is indicated by ___ DNA.

amplified

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Modern Techniques in Microbiology

The process of forming a double-stranded molecule from single strands of DNA or RNA is called ___ hybridization.

nucleic acid

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Modern Techniques in Microbiology

In nucleic acid hybridization, the first step involves ___ to separate the strands.

heating

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Modern Techniques in Microbiology

After combining single strands of DNA, the next step is to ___ to allow renaturation.

cool

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Modern Techniques in Microbiology

Complete hybridization indicates that the organisms are ___.

identical

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Modern Techniques in Microbiology

Partial hybridization suggests that the organisms are ___.

related

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Modern Techniques in Microbiology

No hybridization indicates that the organisms are ___.

unrelated

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Modern Techniques in Microbiology

A DNA chip can contain hundreds of thousands of synthetic single-stranded DNA sequences unique to different ___ species.

bacterial

p.29
Microbial Taxonomy and Classification

Eucaryotes can be ___ or ___ organisms and have nuclei and membrane-bound organelles.

unicellular, multicellular

p.29
Microbial Taxonomy and Classification

Viruses are acellular, parasitic particles composed of ___ and ___.

nucleic acid, protein

p.30
Microbial Taxonomy and Classification

Viruses do not have a ___ membrane.

nuclear

p.30
Microbial Taxonomy and Classification

Bacteria usually have a rigid ___ as their external cell wall.

peptidoglycan

p.30
Microbial Taxonomy and Classification

Fungi have a rigid ___ as their external cell wall.

chitin

p.30
Microbial Taxonomy and Classification

Antibiotic sensitivity is present in ___ but not in viruses or worms.

bacteria

p.30
Microbial Taxonomy and Classification

Protozoa can reproduce ___ and outside host cells.

within

p.31
Microbial Taxonomy and Classification

Viruses can be classified based on their ___ and arrangement of nucleic acid.

nucleic acid

p.31
Microbial Taxonomy and Classification

The family of the virus that has a double strand of nucleic acid and an icosahedral symmetry is ___ and the species is ___.

herpesviridae, herpes simplex

p.31
Microbial Taxonomy and Classification

The virus with a double strand of nucleic acid, no envelope, and complex symmetry belongs to the family ___ and the species is ___.

poxviridae, vaccinia virus

p.31
Microbial Taxonomy and Classification

The family of the virus that has a single strand of nucleic acid and no envelope is ___ and the species is ___.

parvoviridae, human parvovirus

p.31
Microbial Taxonomy and Classification

The structure of the virus particle can be classified as ___ or ___ based on the presence of an envelope.

envelope, no envelope

p.32
Microbial Taxonomy and Classification

Procaryotes are measured in ___ meters.

micrometers

p.32
Microbial Taxonomy and Classification

Viruses are measured in ___ meters.

nanometers

p.32
Microbial Taxonomy and Classification

Helminths are measured in ___ meters.

millimeters

p.8
Key Figures in Microbiology

___ and ___ each demonstrated the presence of heat resistant forms of some microbes.

John Tyndall, Ferdinand Cohn

p.8
Microbial Taxonomy and Classification

Cohn determined these heat resistant forms to be ___.

endospores

p.9
Aseptic Techniques and Sterilization

Dr. Oliver Wendell Holmes noted that mothers of home births had ___ infections than those in hospitals.

fewer

p.29
Microbial Taxonomy and Classification

Procaryotes are microscopic, unicellular organisms that lack ___ and ___ organelles.

nuclei, membrane-bound

p.3
Introduction to Microbiology

Microbiology studies ___ which are too small to be seen with the naked eye.

microorganisms

p.3
Introduction to Microbiology

To view microorganisms, a ___ is needed.

microscope

p.3
Introduction to Microbiology

Microorganisms include bacteria, viruses, fungi, protozoa, ___, and algae.

helminths (worms)

p.4
Importance of Microorganisms

Microorganisms contribute to nutrient production and energy flow by producing ___, ___, ___, ___, ___, and ___.

wine, vinegar, beer, bread, yogurt, cheese

p.4
Importance of Microorganisms

Microbes play a crucial role in the ___ of organic waste.

decomposition

p.4
Importance of Microorganisms

In biotechnology and genetic engineering, microbes are used to produce ___, ___, ___, and ___.

vaccines, antibiotics, interferons, insulin

p.4
Importance of Microorganisms

Microorganisms can harm us by causing ___ disease.

infectious

p.5
Infectious Diseases and Their Impact

Approximately ___ different microbes are known to cause diseases.

2000

p.5
Infectious Diseases and Their Impact

There are about ___ billion new infections worldwide each year.

10

p.5
Infectious Diseases and Their Impact

The number of deaths from infections worldwide each year is approximately ___ million.

13

p.5
Infectious Diseases and Their Impact

___% of deaths are caused by respiratory infections, making it the largest contributor to infectious disease mortality.

26

p.5
Infectious Diseases and Their Impact

___% of deaths are attributed to AIDS, highlighting its significant impact on global health.

18

p.6
Historical Development of Microbiology

The historical development of microbiology spans over ___ years of contributions by many researchers.

300

p.6
Historical Development of Microbiology

Prominent discoveries in microbiology include microscopy, the scientific method, and the development of ___ microbiology.

medical

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Historical Development of Microbiology

One of the key discoveries in the historical development of microbiology is the advancement of ___ techniques.

microbiology

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Key Figures in Microbiology

Antonie van Leeuwenhoek was a Dutch ___ merchant.

linen

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Key Figures in Microbiology

He was the first to observe ___ microbes.

living

p.7
Key Figures in Microbiology

Leeuwenhoek's single-lens microscope could magnify objects up to ___X.

300

p.8
Aseptic Techniques and Sterilization

___ requires the elimination of all life forms including endospores and viruses.

Sterility

p.26
Modern Techniques in Microbiology

The process of inserting unknown DNA into a chip allows it to ___ with the DNA on the chip.

hybridize

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Modern Techniques in Microbiology

The unknown DNA from a patient is separated into single strands, enzymatically cut, and labeled with a ___ dye.

fluorescent

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Modern Techniques in Microbiology

The tagged DNA will bind only to the ___ DNA on the chip.

complementary

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Modern Techniques in Microbiology

In the DNA microarray, red indicates a gene expressed in ___ cells, while green indicates a mutated gene expressed in ___ cells.

normal, tumor

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Modern Techniques in Microbiology

In Fluorescent In Situ Hybridization (FISH), a ___ or ___ probe is added that is attached to a fluorescent dye.

DNA, RNA

p.27
Modern Techniques in Microbiology

FISH can be used to identify ___ species, such as S. aureus.

bacterial

p.28
Microbial Taxonomy and Classification

In binomial nomenclature, each microbe has two names: the ___ which is always capitalized, and the ___ which is lowercase.

Genus, species

p.28
Microbial Taxonomy and Classification

Both the genus and species names in bacterial nomenclature are ___ or ___ .

italicized, underlined

p.28
Microbial Taxonomy and Classification

An example of a bacterium is ___ ___ (S. aureus), where the first part is the genus and the second part is the species.

Staphylococcus aureus

p.28
Microbial Taxonomy and Classification

The species name in the binomial nomenclature is always written in ___ case.

lower

p.29
Microbial Taxonomy and Classification

Microorganisms can be classified into ___ and ___.

procaryotes, eucaryotes

p.9
Aseptic Techniques and Sterilization

Dr. Ignaz Semmelweis linked infections in maternity wards to physicians coming directly from the ___ room.

autopsy

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