Joseph Lister introduced aseptic techniques that involved disinfection of hands using ___ prior to surgery.
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chemicals
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Joseph Lister introduced aseptic techniques that involved disinfection of hands using ___ prior to surgery.
chemicals
Joseph Lister's techniques also included the use of ___ for sterilization.
heat
Many diseases are caused by the growth of ___ in the body and not by sins, bad character, or poverty, etc.
microbes
The two major contributors to the germ theory of disease are ___ and ___.
Louis Pasteur, Robert Koch
Louis Pasteur showed that ___ caused fermentation and spoilage.
microbes
Louis Pasteur disproved the theory of ___ generation of microorganisms.
spontaneous
Louis Pasteur developed a process known as ___.
pasteurization
Louis Pasteur demonstrated what is now known as the ___ Theory of Disease.
Germ
Louis Pasteur developed a vaccine for ___.
rabies
Robert Koch established ___'s postulates, a sequence of experimental steps that verified the germ theory.
Koch
Robert Koch identified the cause of ___, TB, and cholera.
anthrax
Robert Koch developed ___ culture methods.
pure
Robert Koch was awarded the Nobel Prize in ___ for his contributions to microbiology.
1905
___ is known for the smallpox vaccination developed in 1796.
Jenner
About ___ years after Jenner, Pasteur demonstrated how vaccinations work.
100
Pasteur's work involved the creation of ___ strains of bacteria during extended laboratory cultivation.
avirulent
In ___, Paul Ehrlich developed a synthetic arsenic drug called salvarsan to treat syphilis.
1910
The synthesis of ___ began in the 1930s.
sulfonamides
In ___, Alexander Fleming discovered the first antibiotic, Penicillin.
1928
___, ___, ___, ___, and ___ are the main branches of microbiology.
Bacteriology, Mycology, Parasitology, Virology, Immunology
Microbial genetics and molecular biology have led to ___ technology.
Recombinant DNA
The prokaryotic model system used in recombinant DNA technology is ___ .
E. coli
In 1901, ___ was awarded the Nobel Prize for the development of Diphtheria antitoxin.
von Behring
The Nobel Prize in 1902 was awarded to ___ for his work on malaria transmission.
Ross
___ discovered the TB bacterium and received the Nobel Prize in 1905.
Koch
The Nobel Prize in 1908 was awarded to ___ for his research on phagocytes.
Metchnikoff
In 1945, the Nobel Prize was awarded to Fleming, Chain, and Florey for their discovery of ___.
Penicillin
___ was awarded the Nobel Prize in 1952 for the discovery of Streptomycin.
Waksman
The Nobel Prize in 1969 was awarded to Delbrück, Hershey, and Luria for their work on ___.
Viral replication
In 1987, ___ received the Nobel Prize for his research on antibody genetics.
Tonegawa
___ was awarded the Nobel Prize in 1997 for his work on prions.
Prusiner
In 2005, Marshall and Warren received the Nobel Prize for their discovery of ___ and its relation to ulcers.
H. pylori
The Nobel Prize in 2008 was awarded to Harald zur Hausen for his research on ___ and cervical cancer.
HPV
Françoise Barré-Sinoussi and Luc Montagnier were awarded the Nobel Prize for their discovery of ___.
HIV
The formal system of taxonomy was originated by ___ ___ ___.
Carl von Linné
Taxonomy is concerned with ___, which is the orderly arrangement of organisms into groups.
classification
In taxonomy, ___ refers to assigning names to organisms.
nomenclature
The process of discovering and recording traits of organisms for placement into taxonomic schemes is known as ___.
identification
Living organisms are classified into three domains: ___, ___, and ___.
Bacteria, Archaea, Eukarya
The Domain Eukarya includes the kingdoms ___, ___, and ___.
Fungi, Plantae, Animalia
All organisms evolved from cells that formed over ___ billion years ago.
3
The DNA passed on from ancestors is described as ___.
conserved
The three domains of life include Bacteria, ___, and Eukarya.
Archaea
___ are true bacteria characterized by peptidoglycan.
Bacteria
Archaea are known for living in ___ environments.
extreme
Eukarya are distinguished by having a ___ and organelles.
nucleus
Morphological characteristics are used for ___ but not for classification.
identification
Differential staining is used for ___ and ___ in bacteria.
classification, identification
Biochemical testing is used for ___ only.
identification
Serology is used for ___ only.
identification
Phage typing is used for ___ only.
identification
Fatty acid profiles are used for ___ only.
identification
Flow cytometry is used for ___ only.
identification
DNA base composition is used for ___ but not for identification.
classification
DNA fingerprinting is used for both ___ and ___.
classification, identification
PCR is used for both ___ and ___.
classification, identification
Nucleic acid hybridization techniques are used for both ___ and ___.
classification, identification
rRNA sequencing is used for ___ but not for identification.
classification
Morphological characteristics are useful for identifying ___.
eukaryotes
The process of ___ staining is used in differential staining.
Gram
Biochemical tests determine the presence of ___ enzymes.
bacterial
In numerical rapid identification, the ID value is determined by adding the numbers from each group of ___.
tests
The organism identified with an ID value of 21006 is ___.
Proteus mirabilis
Serology involves reactions of microorganisms with specific antibodies: ___ known anti-serum with unknown bacterium.
Combine
Serology is useful in determining the identity of strains and species, as well as ___ among organisms.
relationships
Phage typing is used for the identification of bacterial species and strains by determining their susceptibility to various ___.
phages
DNA fingerprinting uses the number and sizes of DNA fragments produced by ___ digests to determine genetic similarities.
RE
___ sequencing is a method used in ribotyping.
rRNA
The ___ can be used to amplify a small amount of microbial DNA in a sample.
Polymerase chain reaction (PCR)
The presence or identification of an organism is indicated by ___ DNA.
amplified
The process of forming a double-stranded molecule from single strands of DNA or RNA is called ___ hybridization.
nucleic acid
In nucleic acid hybridization, the first step involves ___ to separate the strands.
heating
After combining single strands of DNA, the next step is to ___ to allow renaturation.
cool
Complete hybridization indicates that the organisms are ___.
identical
Partial hybridization suggests that the organisms are ___.
related
No hybridization indicates that the organisms are ___.
unrelated
A DNA chip can contain hundreds of thousands of synthetic single-stranded DNA sequences unique to different ___ species.
bacterial
Eucaryotes can be ___ or ___ organisms and have nuclei and membrane-bound organelles.
unicellular, multicellular
Viruses are acellular, parasitic particles composed of ___ and ___.
nucleic acid, protein
Viruses do not have a ___ membrane.
nuclear
Bacteria usually have a rigid ___ as their external cell wall.
peptidoglycan
Fungi have a rigid ___ as their external cell wall.
chitin
Antibiotic sensitivity is present in ___ but not in viruses or worms.
bacteria
Protozoa can reproduce ___ and outside host cells.
within
Viruses can be classified based on their ___ and arrangement of nucleic acid.
nucleic acid
The family of the virus that has a double strand of nucleic acid and an icosahedral symmetry is ___ and the species is ___.
herpesviridae, herpes simplex
The virus with a double strand of nucleic acid, no envelope, and complex symmetry belongs to the family ___ and the species is ___.
poxviridae, vaccinia virus
The family of the virus that has a single strand of nucleic acid and no envelope is ___ and the species is ___.
parvoviridae, human parvovirus
The structure of the virus particle can be classified as ___ or ___ based on the presence of an envelope.
envelope, no envelope
Procaryotes are measured in ___ meters.
micrometers
Viruses are measured in ___ meters.
nanometers
Helminths are measured in ___ meters.
millimeters
___ and ___ each demonstrated the presence of heat resistant forms of some microbes.
John Tyndall, Ferdinand Cohn
Cohn determined these heat resistant forms to be ___.
endospores
Dr. Oliver Wendell Holmes noted that mothers of home births had ___ infections than those in hospitals.
fewer
Procaryotes are microscopic, unicellular organisms that lack ___ and ___ organelles.
nuclei, membrane-bound
Microbiology studies ___ which are too small to be seen with the naked eye.
microorganisms
To view microorganisms, a ___ is needed.
microscope
Microorganisms include bacteria, viruses, fungi, protozoa, ___, and algae.
helminths (worms)
Microorganisms contribute to nutrient production and energy flow by producing ___, ___, ___, ___, ___, and ___.
wine, vinegar, beer, bread, yogurt, cheese
Microbes play a crucial role in the ___ of organic waste.
decomposition
In biotechnology and genetic engineering, microbes are used to produce ___, ___, ___, and ___.
vaccines, antibiotics, interferons, insulin
Microorganisms can harm us by causing ___ disease.
infectious
Approximately ___ different microbes are known to cause diseases.
2000
There are about ___ billion new infections worldwide each year.
10
The number of deaths from infections worldwide each year is approximately ___ million.
13
___% of deaths are caused by respiratory infections, making it the largest contributor to infectious disease mortality.
26
___% of deaths are attributed to AIDS, highlighting its significant impact on global health.
18
The historical development of microbiology spans over ___ years of contributions by many researchers.
300
Prominent discoveries in microbiology include microscopy, the scientific method, and the development of ___ microbiology.
medical
One of the key discoveries in the historical development of microbiology is the advancement of ___ techniques.
microbiology
Antonie van Leeuwenhoek was a Dutch ___ merchant.
linen
He was the first to observe ___ microbes.
living
Leeuwenhoek's single-lens microscope could magnify objects up to ___X.
300
___ requires the elimination of all life forms including endospores and viruses.
Sterility
The process of inserting unknown DNA into a chip allows it to ___ with the DNA on the chip.
hybridize
The unknown DNA from a patient is separated into single strands, enzymatically cut, and labeled with a ___ dye.
fluorescent
The tagged DNA will bind only to the ___ DNA on the chip.
complementary
In the DNA microarray, red indicates a gene expressed in ___ cells, while green indicates a mutated gene expressed in ___ cells.
normal, tumor
In Fluorescent In Situ Hybridization (FISH), a ___ or ___ probe is added that is attached to a fluorescent dye.
DNA, RNA
FISH can be used to identify ___ species, such as S. aureus.
bacterial
In binomial nomenclature, each microbe has two names: the ___ which is always capitalized, and the ___ which is lowercase.
Genus, species
Both the genus and species names in bacterial nomenclature are ___ or ___ .
italicized, underlined
An example of a bacterium is ___ ___ (S. aureus), where the first part is the genus and the second part is the species.
Staphylococcus aureus
The species name in the binomial nomenclature is always written in ___ case.
lower
Microorganisms can be classified into ___ and ___.
procaryotes, eucaryotes
Dr. Ignaz Semmelweis linked infections in maternity wards to physicians coming directly from the ___ room.
autopsy