What factors affect the rate of diffusion?
Concentration, temperature, and pressure.
What does the nucleus contain and control?
Contains chromosomes which carry genetic information and controls the activities of the cell.
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p.5
Diffusion and Osmosis

What factors affect the rate of diffusion?

Concentration, temperature, and pressure.

p.1
Cell Structure and Function

What does the nucleus contain and control?

Contains chromosomes which carry genetic information and controls the activities of the cell.

p.2
Microscopy Techniques

What is the maximum magnification achievable with a light microscope?

x1000

p.6
Cell Structure and Function

What is a selectively permeable membrane?

A membrane that allows certain molecules to pass through but not others.

p.1
Cell Structure and Function

What is the function of the cell wall in plant cells?

Contains cellulose; it supports the plant’s structure.

p.6
Cell Structure and Function

How does the cell membrane control movement in and out of cells?

The cell membrane is selectively permeable, allowing certain molecules to pass through while blocking others.

p.3
Microscopy Techniques

What should you do with the cotton bud after wiping it in the methylene blue?

Place the cotton bud in a beaker of disinfectant.

p.6
Cell Structure and Function

Why can't large molecules pass through the cell membrane?

Large molecules are too big to pass through the pores in the membrane.

p.3
Microscopy Techniques

What is the final step in examining cheek cells using a light microscope?

Use a light microscope to examine the slide.

p.5
Diffusion and Osmosis

What is diffusion?

The process where molecules move from an area of high concentration to an area of low concentration without requiring energy.

p.1
Cell Structure and Function

Where do most chemical reactions take place in a cell?

In the cytoplasm.

p.5
Diffusion and Osmosis

How does pressure affect the rate of diffusion?

If there is high pressure, molecules will quickly move from the area of high pressure to low pressure.

p.3
Microscopy Techniques

How do you collect cheek cells for examination?

Gently rub the inside of your cheek with a cotton bud.

p.5
Diffusion and Osmosis

Why do molecules of liquids and gases constantly move and mix?

Because they have kinetic energy.

p.9
Diffusion and Osmosis

What happens to animal cells placed in a concentrated solution?

Water moves out of the cell through the selectively permeable cell membrane, causing the cell to shrivel.

p.2
Microscopy Techniques

Why can you only study dead cells with an electron microscope?

Because the process of using electron microscopes requires cells to be dead.

p.2
Microscopy Techniques

What did electron microscopes allow scientists to discover?

The internal structures of cells.

p.1
Cell Structure and Function

What is the function of the cell membrane?

Controls substances entering and leaving the cell.

p.5
Diffusion and Osmosis

How does concentration affect the rate of diffusion?

The greater the difference in concentration between two areas (the concentration gradient), the faster the rate of diffusion.

p.1
Cell Structure and Function

What is the role of chloroplasts in plant cells?

Absorb light for photosynthesis.

p.6
Cell Structure and Function

What types of molecules can pass through the pores in the cell membrane?

Small molecules, such as water.

p.3
Microscopy Techniques

How do you prepare the slide for examination after adding the sample?

Use a mounted needle to gently lower a coverslip onto the glass slide.

p.4
Specialized Cells and Their Functions

What are unicellular organisms?

The simplest forms of living organisms made of only one cell.

p.2
Microscopy Techniques

How do light microscopes allow you to see an image?

Light passes through the image.

p.1
Cell Structure and Function

What is the function of the vacuole in plant cells?

Space filled with cell sap (a dilute solution of sugars and mineral salts).

p.2
Microscopy Techniques

What does an electron microscope use instead of light?

A beam of electrons.

p.7
Diffusion and Osmosis

What is osmosis?

Osmosis is the diffusion of water molecules from an area of high water concentration (low solute concentration) to an area of low water concentration (high solute concentration) through a selectively permeable membrane.

p.9
Diffusion and Osmosis

What happens to plant cells placed in a dilute solution?

Water moves into the cell through the selectively permeable cell membrane, causing the cell to become turgid. The cell wall prevents it from bursting.

p.1
Differences Between Plant and Animal Cells

What type of vacuole is present in plant cells but not in animal cells?

Large permanent vacuole.

p.2
Biological Staining Methods

What is the purpose of biological staining?

To help us see cells in greater detail.

p.9
Diffusion and Osmosis

Why do plant cells become turgid in a dilute solution?

Plant cells become turgid because water moves into the cell, and the cell wall prevents it from bursting.

p.7
Diffusion and Osmosis

What happens to water molecules during osmosis according to Fig. 1?

Water molecules move down a concentration gradient from an area of high water concentration to an area of low water concentration through a selectively permeable membrane.

p.7
Diffusion and Osmosis

What happens when there is no net movement of water according to Fig. 2?

The concentration of water on both sides of the membrane is equal, and the same numbers of water molecules move in both directions.

p.2
Microscopy Techniques

What is the total magnification for a low power eyepiece lens (x10) and objective lens (x4)?

x40

p.8
Diffusion and Osmosis

Why does water move into the Visking tubing in Investigation 1?

Because the concentration of water outside the Visking tubing is higher than inside, causing water to move in by osmosis.

p.4
Levels of Organization in Multicellular Organisms

What is an organ system?

A number of organs working together to carry out specific functions.

p.13
Factors Affecting Enzyme Activity

What is the optimum pH for enzyme activity?

The pH at which enzymes work best.

p.12
Enzyme Structure and Function

What is the active site of an enzyme?

The active site of an enzyme is a cleft in the protein where a specific substrate 'fits'.

p.11
Active Transport Mechanisms

Why is energy required for active transport?

Because materials are moved against their concentration gradient, from low to high concentration.

p.11
Active Transport Mechanisms

Give an example of active transport in plants.

Uptake of nitrate ions by root hair cells from the soil water.

p.14
Factors Affecting Enzyme Activity

What happens to an enzyme when it is denatured?

The enzyme can no longer bind with its substrate, and no reaction can occur.

p.5
Diffusion and Osmosis

How does temperature affect the rate of diffusion?

As the temperature increases, the rate of diffusion increases because molecules have more kinetic energy.

p.5
Diffusion and Osmosis

What types of molecules pass through the cell membrane by diffusion?

Oxygen and carbon dioxide.

p.1
Cell Structure and Function

Where does aerobic respiration occur in the cell?

In the mitochondria.

p.9
Diffusion and Osmosis

What happens to animal cells placed in a dilute solution?

Water moves into the cell through the selectively permeable cell membrane, causing the cell to swell and potentially burst because there is no cell wall.

p.6
Cell Structure and Function

What is an example of a large molecule that cannot pass through the cell membrane?

Minerals and sugars.

p.7
Diffusion and Osmosis

What is a dilute solution in terms of solute and water concentration?

A dilute solution has a low solute concentration and therefore a high water concentration.

p.7
Diffusion and Osmosis

What is a concentrated solution in terms of solute and water concentration?

A concentrated solution has a high solute concentration and therefore a low water concentration.

p.6
Cell Structure and Function

What must every substance that enters or leaves a cell cross?

The cell membrane.

p.6
Cell Structure and Function

What does the structure of the cell membrane appear to have?

Many pores in its surface.

p.2
Biological Staining Methods

What does methylene blue stain and what is it mostly used for?

It stains acidic parts of the cell blue and makes the nucleus more obvious. Mostly used to prepare slides of animal cells and bacteria.

p.8
Diffusion and Osmosis

What happens to the solution inside the Visking tubing in Investigation 1?

The solution moves up the capillary tube due to increased pressure inside the Visking tubing.

p.2
Microscopy Techniques

What is the total magnification for a high power eyepiece lens (x10) and objective lens (x20)?

x200

p.8
Diffusion and Osmosis

What happens to Tube 1 in Investigation 2?

Tube 1 gets bigger (becomes turgid) because water moves in through the selectively permeable membrane by osmosis.

p.10
Diffusion and Osmosis

Why are the ends of the Visking tubes tied in Investigation 4?

To prevent the solution from leaking out.

p.13
Enzyme Structure and Function

What are metabolic reactions?

Reactions that involve building large molecules from small ones as well as breaking down large molecules into smaller ones.

p.11
Active Transport Mechanisms

What is active transport?

The process of moving materials from an area of low concentration to an area of high concentration, requiring energy.

p.11
Active Transport Mechanisms

Why can't nitrate ions move into plant cells by diffusion?

Because the concentration of nitrate ions is higher inside the plant cells than in the soil water.

p.11
Active Transport Mechanisms

How do marine algae use active transport?

They concentrate iodine in their cells to levels a million times greater than the surrounding sea water.

p.11
Active Transport Mechanisms

How does oxygen affect active transport?

Aerobic respiration needs oxygen to produce ATP, which is required for active transport.

p.14
Factors Affecting Enzyme Activity

Can enzymes A and B work at the same pH?

Yes, both enzyme A and B can work at the same pH, but their rate of reaction may be below their optimum.

p.3
Biological Staining Methods

What is the first step in examining cheek cells using a light microscope?

Put a drop of methylene blue on a glass slide.

p.2
Microscopy Techniques

When was the electron microscope developed?

In the 1930s.

p.1
Differences Between Plant and Animal Cells

What is a key difference between plant and animal cells regarding the cell wall?

Plant cells have a cell wall, while animal cells do not.

p.2
Microscopy Techniques

What is the maximum magnification achievable with an electron microscope?

Up to x50,000,000.

p.6
Cell Structure and Function

What is an example of a small molecule that can pass through the cell membrane?

Water.

p.6
Cell Structure and Function

What is Visking tubing used for in the context of cell membranes?

Visking tubing is used to model how the cell membrane works.

p.8
Cell Structure and Function

What is Visking tubing similar to in living organisms?

The cell membrane.

p.8
Cell Structure and Function

What type of membrane is Visking tubing?

Selectively permeable membrane.

p.4
Specialized Cells and Their Functions

Give an example of a specialized cell and its function.

Red blood cells carry oxygen.

p.2
Microscopy Techniques

How is total magnification achieved in a microscope?

By multiplying the power of the eyepiece lens by the power of the objective lens.

p.4
Levels of Organization in Multicellular Organisms

What is an organ?

Several tissues performing specific functions.

p.13
Factors Affecting Enzyme Activity

What is the optimum temperature for enzyme activity?

The temperature at which enzymes work best.

p.8
Diffusion and Osmosis

What happens to Tube 2 in Investigation 2?

Tube 2 gets smaller (becomes flaccid) because water moves out through the selectively permeable membrane by osmosis.

p.12
Enzyme Structure and Function

What determines the active site of an enzyme?

The active site of an enzyme depends on the shape of the protein, which is held by chemical bonds.

p.10
Diffusion and Osmosis

What does it indicate if the mass of the potato cylinders or Visking tubes has not changed?

The concentration of the solution inside the potato cells or Visking tubes is equal to the concentration of the solution outside.

p.10
Diffusion and Osmosis

What does it indicate if the mass of the potato cylinders or Visking tubes has decreased?

The concentration of water outside the cylinder or bag is lower than the concentration inside, causing water to move out through osmosis.

p.14
Factors Affecting Enzyme Activity

What is the effect of high temperatures on enzymes?

High temperatures cause the specific shape of the active site to be destroyed, denaturing the enzyme and preventing it from binding with its substrate.

p.14
Factors Affecting Enzyme Activity

What happens to enzyme activity when the pH is far below or above the optimum?

The enzyme is denatured, and no reaction can occur.

p.1
Differences Between Plant and Animal Cells

Do animal cells have chloroplasts?

No, animal cells do not have chloroplasts.

p.7
Cell Structure and Function

What type of membrane is the cell membrane?

The cell membrane is a selectively permeable membrane; it lets some molecules through but not others.

p.3
Microscopy Techniques

How do you prepare onion cells for examination using a light microscope?

Peel a thin layer of epidermis from the inside of a freshly cut piece of onion and lay it onto a glass slide.

p.3
Biological Staining Methods

What staining solution is used for examining onion cells?

Iodine solution.

p.7
Cell Structure and Function

What do the pores in the selectively permeable membrane allow?

The pores in the membrane allow small water molecules to pass through, but the solutes are too large to pass through the pores.

p.8
Cell Structure and Function

What does Visking tubing allow through its pores?

Small molecules.

p.13
Enzyme Structure and Function

What is the primary function of enzymes?

To speed up/catalyse the rate of a chemical reaction.

p.12
Enzyme Structure and Function

What are some important functions of proteins?

Proteins function as enzymes, hormones (e.g., insulin), and muscle tissue.

p.4
Levels of Organization in Multicellular Organisms

Give an example of an organ system and its function.

The circulatory system carries substances around the body.

p.8
Diffusion and Osmosis

Why does Tube 2 become flaccid in Investigation 2?

Because the concentration of water inside the Visking tubing is higher than outside, causing water to move out by osmosis.

p.13
Enzyme Structure and Function

What happens to an enzyme at the end of a reaction?

The enzyme remains unchanged.

p.14
Factors Affecting Enzyme Activity

What happens to enzyme activity when kinetic energy is low?

There are fewer collisions between the enzyme and substrate, resulting in a lower rate of reaction.

p.11
Active Transport Mechanisms

Give an example of active transport in humans.

Glucose actively transported from the small intestine into the blood.

p.14
Factors Affecting Enzyme Activity

What is the optimum temperature for enzyme activity?

The optimum temperature is the temperature at which the rate of reaction is highest before the enzyme starts to denature.

p.9
Diffusion and Osmosis

What happens to plant cells placed in a concentrated solution?

Water moves out of the cell through the selectively permeable cell membrane, causing the cell to become flaccid. It doesn't shrivel because it has a cell wall.

p.9
Differences Between Plant and Animal Cells

Why do animal cells burst in a dilute solution but plant cells do not?

Animal cells burst because they lack a cell wall, while plant cells do not burst because their cell wall provides structural support.

p.3
Microscopy Techniques

What is the first step in calculating the magnification of a drawing or picture?

Measure the length of A – B on the drawing in millimeters (mm).

p.12
Enzyme Structure and Function

How are the activities of a cell controlled?

All the activities of a cell depend on chemical reactions, which are controlled by special molecules called enzymes.

p.13
Enzyme Structure and Function

What type of biological molecule are enzymes?

Proteins.

p.4
Levels of Organization in Multicellular Organisms

What are tissues?

Groups of similar cells with a similar function.

p.13
Enzyme Structure and Function

What model describes how enzymes work?

The lock and key model.

p.10
Diffusion and Osmosis

What is the purpose of using identical Visking tubes in Investigation 4?

To ensure that each bag contains the same volume (10 ml) of a sugar (or salt) solution for a fair comparison.

p.12
Enzyme Structure and Function

Why is the sequence of amino acids important?

The sequence of amino acids is important in determining the protein structure and its function.

p.10
Diffusion and Osmosis

Why are the potato cylinders dried and weighed before and after the experiment in Investigation 3?

To measure the % change in mass, allowing for a fair comparison.

p.10
Diffusion and Osmosis

What conclusion can be drawn from both investigations regarding osmosis?

Mass changes due to osmosis, where water moves through a selectively permeable membrane from a region of lower solute concentration to a region of higher solute concentration.

p.10
Diffusion and Osmosis

What does it indicate if the mass of the potato cylinders or Visking tubes has increased?

The concentration of water outside the cylinder or bag is higher than the concentration inside, causing water to move in through osmosis.

p.11
Active Transport Mechanisms

What factors affect the rate of active transport?

Glucose concentration, oxygen availability, temperature, and presence of toxic substances.

p.14
Factors Affecting Enzyme Activity

What is the optimum pH for enzymes found in the stomach?

The optimum pH for enzymes in the stomach is pH 2.

p.4
Specialized Cells and Their Functions

What are multicellular organisms?

Organisms that consist of many cells.

p.4
Specialized Cells and Their Functions

Approximately how many cells are human beings made up of?

Approximately 34 to 37 trillion cells.

p.4
Specialized Cells and Their Functions

Why do cells specialize?

To carry out specific functions more efficiently than non-specialized cells.

p.3
Microscopy Techniques

How do you convert millimeters to micrometers?

Multiply the length in millimeters by 1000 (1 mm = 1000 μm).

p.3
Microscopy Techniques

How do you calculate the magnification of a picture?

Divide the length of A – B on the picture by the real length of A – B.

p.12
Enzyme Structure and Function

What are enzymes?

Enzymes are proteins.

p.4
Levels of Organization in Multicellular Organisms

Give an example of an organ and its function.

The heart pumps blood.

p.12
Enzyme Structure and Function

How is the specific shape of a protein formed?

The amino acid chain is folded to form a specific shape.

p.8
Diffusion and Osmosis

Why does Tube 1 become turgid in Investigation 2?

Because the concentration of water outside the Visking tubing is higher than inside, causing water to move in by osmosis.

p.13
Enzyme Structure and Function

What is an enzyme-substrate complex?

A complex formed when a substrate is held in an enzyme's active site, increasing the probability that a reaction will take place.

p.10
Diffusion and Osmosis

Why is it important to calculate the % change in mass in these investigations?

Because all bags and cylinders have different initial masses, calculating the % change allows for a fair comparison.

p.11
Active Transport Mechanisms

How does glucose concentration affect active transport?

Respiration needs glucose to produce ATP, which is required for active transport.

p.14
Factors Affecting Enzyme Activity

What is the optimum pH for enzymes found in the small intestine?

The optimum pH for enzymes in the small intestine is pH 10.

p.1
Levels of Organization in Multicellular Organisms

What determines the size of an organism?

The number of cells, not the size of the cells.

p.2
Biological Staining Methods

What does iodine solution stain and what is it mostly used for?

It makes the nucleus more obvious and stains any starch present. Mostly used to stain plant cells.

p.9
Diffusion and Osmosis

Why do plant cells become flaccid in a concentrated solution?

Plant cells become flaccid because water moves out of the cell, but the cell wall prevents it from shriveling.

p.4
Specialized Cells and Their Functions

What is the function of muscle cells?

Muscle cells contract and therefore shorten.

p.13
Enzyme Structure and Function

Are enzymes specific or non-specific in their action?

Specific; they can only catalyse one type of molecule.

p.12
Enzyme Structure and Function

What are proteins made of?

Proteins are made of different amino acids linked together to form a chain.

p.13
Factors Affecting Enzyme Activity

What happens to enzymes if the temperature is too high?

The enzyme’s active site will change shape and stop working, a process called denaturation.

p.10
Diffusion and Osmosis

What is the significance of drying and weighing the bags before and after the experiment in Investigation 4?

To measure the % change in mass, allowing for a fair comparison.

p.10
Diffusion and Osmosis

What is the common variable between Investigation 3 and Investigation 4?

Both investigations measure the % change in mass after being placed in different concentrations of a solution for the same length of time (30 minutes).

p.11
Active Transport Mechanisms

What provides the energy required for active transport?

Respiration provides the energy in the form of ATP.

p.14
Factors Affecting Enzyme Activity

How does increased kinetic energy affect enzyme activity?

Enzyme and substrate have more kinetic energy and collide more often, increasing the rate of reaction.

p.14
Factors Affecting Enzyme Activity

How does pH affect enzyme activity?

The rate of reaction varies according to the pH level. Enzymes have an optimum pH at which they function best, and deviations from this pH can denature the enzyme.

p.11
Active Transport Mechanisms

How do toxic substances like cyanide affect active transport?

Cyanide stops respiration, which halts ATP production and thus active transport.

p.11
Active Transport Mechanisms

How does temperature affect active transport?

Temperature affects the enzymes controlling respiration, which in turn affects ATP production and active transport.

Study Smarter, Not Harder
Study Smarter, Not Harder