In what type of reactions is water a reactant?
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In many of the decomposition reactions of digestion.
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In what type of reactions is water a reactant?
In many of the decomposition reactions of digestion.
What role does water play in temperature regulation?
It acts as an excellent temperature buffer.
What does an acid dissociate into?
H+ and anions.
What does a base dissociate into?
OH- and cations.
What does a salt dissociate into?
Negative and positive ions, neither of which is H+ or OH-.
What does the term pH refer to?
The concentration of H+ in a solution.
What is the pH of a neutral solution?
pH 7.
What stabilizes the pH inside a cell and in culture media?
pH buffers.
What do organic compounds always contain?
Carbon and hydrogen.
How many bonds can carbon atoms form with other atoms?
Up to four bonds.
What type of bonding is mostly found in organic compounds?
Covalently bonded.
What forms a carbon skeleton?
A chain of carbon atoms.
What is ATP composed of?
An adenosine unit and three phosphate groups.
What happens when the bond to ATP's terminal phosphate group is hydrolyzed?
Energy is released.
What is an ionic bond?
A chemical attraction between ions of opposite charge.
What is a covalent bond?
A bond where atoms share pairs of electrons.
What is a hydrogen bond?
A bond that exists when a hydrogen atom covalently bonded to one oxygen or nitrogen atom is attracted to another oxygen or nitrogen atom.
What is molecular mass?
The sum of the atomic masses of all the atoms in a molecule.
What are chemical reactions?
The making or breaking of chemical bonds between atoms.
What is an endergonic reaction?
A reaction that requires more energy than it releases.
What is a synthesis reaction?
A reaction where atoms, ions, or molecules are combined to form a larger molecule.
What is a decomposition reaction?
A reaction where a larger molecule is broken down into its component molecules, ions, or atoms.
What is an exchange reaction?
A reaction where two molecules are decomposed, and their subunits are used to synthesize two new molecules.
What are inorganic compounds typically characterized by?
They are usually small, ionically bonded molecules.
What is the most abundant substance in cells?
Water.
What do microorganisms use nutrients for?
To make chemical building blocks for growth and other essential functions of life.
What is required for microorganisms to synthesize building blocks?
They need to break down nutrient substances and use the energy released to assemble molecular fragments into new substances.
What are some everyday examples of microbial chemical reactions?
A fallen tree rotting on the forest floor and milk going sour in the refrigerator.
Why is knowledge of chemistry essential for microbiologists?
To understand the roles microorganisms play in nature, how they cause disease, and how methods for diagnosing disease are developed.
What unique feature does Bacillus anthracis bacteria have that helps it avoid host defenses?
It makes a capsule that is not readily digested by animal cells.
What toxin do Bacillus anthracis bacteria produce?
A toxin that can harm the host and is being researched for detection in biological weapons.
What do researchers investigate regarding Bacillus anthracis?
Unique chemicals made by Bacillus anthracis to detect the manufacture or use of biological weapons.
What is the significance of understanding how molecules are formed and interact in microbiology?
To understand the changes that occur in microorganisms and the changes microbes make in the world around us.
How should a health advisory nurse respond to a man concerned about his blood sugar levels not decreasing with organic sugar?
The nurse should explain the importance of understanding biological molecules and how they affect blood sugar levels, possibly suggesting further reading or consultation with a healthcare provider.
What is the smallest component of a substance that cannot be subdivided without losing its properties?
An atom.
What are the negatively charged particles that move around the nucleus of an atom called?
Electrons.
What is the central part of an atom called?
The nucleus.
What particles make up the nucleus of an atom?
Protons and neutrons.
What is the charge of the nucleus of an atom?
Net positive charge.
What is the relationship between the number of protons and electrons in an atom?
They are equal, making the atom electrically neutral.
What is the atomic number of an element?
The number of protons in the nucleus.
What are isotopes?
Atoms with different numbers of neutrons in their nuclei but the same number of protons.
How are chemical elements classified?
By the number of protons they contain.
What is the approximate atomic mass of protons and neutrons compared to electrons?
Protons and neutrons are about 1840 times heavier than electrons.
In a water molecule (H2O), which atom has a slightly negative charge?
The oxygen atom.
What is the formula to calculate the pH of a solution?
pH is calculated as -log10[H+].
What is the difference between acidic and basic solutions in terms of H+ and OH- concentrations?
Acidic solutions have more H+ than OH- and a pH lower than 7; basic solutions have more OH- than H+ and a pH above 7.
What are the building blocks of carbohydrates?
Monosaccharides.
What distinguishes simple lipids from complex lipids?
Simple lipids are primarily made of glycerol and fatty acids, while complex lipids contain additional components such as phosphates or sugars.
What are the building blocks of proteins?
Amino acids.
What are the building blocks of nucleic acids?
Nucleotides.
What is the role of ATP in cellular activities?
ATP serves as the primary energy currency of the cell, providing energy for various biochemical processes.
What percentage of living cells is constituted by inorganic compounds, excluding water?
About 1-1.5%.
What is the role of carbon's four outer electrons in organic molecules?
They can participate in up to four covalent bonds.
What is the carbon skeleton in organic molecules?
The chain of carbon atoms in an organic molecule.
What are functional groups in organic compounds?
Specific groups of atoms that are most commonly involved in chemical reactions and are responsible for the characteristic properties of organic compounds.
What is the biological importance of the hydroxyl group (-OH)?
It is hydrophilic and helps dissolve organic molecules containing hydroxyl groups.
What properties do carboxyl groups confer on organic molecules?
They have acidic properties because they are a source of hydrogen ions.
How do amino groups function in organic compounds?
They function as bases because they readily accept hydrogen ions.
What is the significance of the sulfhydryl group in proteins?
It helps stabilize the intricate structure of many proteins.
What is the formula for the hydroxyl group?
-OH.
What type of compounds are classified as alcohols?
Compounds that contain the -OH (hydroxyl) group.
What does the letter R represent in organic chemistry?
The remainder of the molecule when characterizing a class of compounds by a certain functional group.
What are macromolecules?
Large molecules formed by covalent bonding of many repeating small molecules called monomers.
What is dehydration synthesis?
A reaction where two monomers join together, releasing a molecule of water.
What is the principal function of carbohydrates in living systems?
To fuel cell activities with a ready source of energy.
What is the ratio of hydrogen to oxygen atoms in simple carbohydrates?
Always 2:1.
What are the building blocks of deoxyribonucleic acid (DNA)?
Sugars, specifically deoxyribose.
Why is water considered an excellent solvent?
Because it is a polar molecule.
What is the significance of the hydrophobic interaction in protein structure?
Hydrophobic interactions contribute to the tertiary structure of proteins by influencing how the polypeptide folds and maintains its shape.
What is the significance of D-forms of amino acids in relation to phagocytes?
Phagocytes cannot easily digest D-forms of amino acids, such as D-glutamic acid found in the capsules of B. anthracis, which can lead to infection.
What are conjugated proteins?
Conjugated proteins are combinations of amino acids with other organic or inorganic components, named by their non-amino acid component.
What are the structural units of nucleic acids?
Nucleotides are the structural units of nucleic acids.
What are the three parts of a nucleotide?
A heterocyclic nitrogen-containing compound, a pentose sugar, and a phosphate group.
What are the two types of nucleic acids?
DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
What is the structure of DNA as proposed by Watson and Crick?
A DNA molecule consists of two long strands wrapped around each other to form a double helix.
What is the role of nitrogen-containing bases in DNA?
They make up the rungs of the ladder in the DNA structure and are paired specifically (A with T, G with C).
What is the significance of the order of nitrogen base pairs in DNA?
The order contains the genetic instructions for the organism and determines hereditary traits.
What is a nucleoside?
A nucleoside refers to the combination of a purine or pyrimidine plus a pentose sugar, without a phosphate group.
What is the pairing rule for nitrogen bases in DNA?
A pairs with T and G pairs with C, held together by hydrogen bonds.
What are the four nitrogenous bases found in DNA?
Adenine, Thymine, Cytosine, and Guanine.
What components make up an individual DNA nucleotide?
A deoxyribose sugar molecule, a phosphate group, and a nitrogen-containing base.
What is the significance of isotopes in biological research?
They are useful in medical diagnosis, treatment of disorders, and sterilization methods.
What are the most abundant chemical elements in living organisms?
Hydrogen, carbon, nitrogen, and oxygen.
What is the atomic number of Carbon?
6
What is the approximate atomic mass of Carbon?
12
What is the maximum number of bonds that Carbon can form?
4
What is the electronic configuration of Oxygen?
6 valence electrons, 2 unfilled spaces, and can form 2 maximum bonds.
What happens when an atom's outer electron shell is filled?
The atom is chemically stable or inert and does not tend to react with other atoms.
What is the atomic number of Hydrogen?
1
What is the atomic mass of Hydrogen?
1
How does 14C differ from 12C?
14C has 2 more neutrons than 12C, making it a radioactive isotope.
What is the atomic number of 14C and 12C?
Both have an atomic number of 6.
What is the atomic mass of 14C?
Approximately 14.
What is the definition of an ionic bond?
An ionic bond is an attraction between ions of opposite charge that holds them together to form a stable molecule.
What is a covalent bond?
A covalent bond is a chemical bond formed by two atoms sharing one or more pairs of electrons.
What is the valence of an atom?
The valence of an atom is the number of extra or missing electrons in its outermost electron shell.
How do atoms achieve a full complement of electrons in their outermost energy shells?
Atoms achieve a full complement of electrons by combining to form molecules.
What is a compound?
A molecule that contains at least two different kinds of atoms, such as H₂O (water).
What happens to an atom when it gains electrons?
It acquires an overall negative charge and becomes a negatively charged ion (anion).
What happens to an atom when it loses electrons?
It acquires an overall positive charge and becomes a positively charged ion (cation).
What is the role of weaker ionic bonds in living cells?
Weaker ionic bonds formed in aqueous solutions are important in biochemical reactions in microbes and other organisms.
What is the difference between cations and anions?
Cations are positively charged ions formed when an atom loses electrons, while anions are negatively charged ions formed when an atom gains electrons.
What is the most chemically stable configuration for any atom?
To have its outermost shell filled with electrons.
What happens to a sodium atom when it loses an electron?
It becomes a sodium ion (Na+).
What is formed when a chlorine atom gains an electron?
It becomes a chloride ion (Cl-).
What type of bond is formed between sodium and chloride ions?
An ionic bond.
What is a covalent bond?
A bond formed by the sharing of electrons between atoms.
What is an example of a molecule formed by covalent bonding?
Methane (CH4).
How do hydrogen and carbon typically form bonds in living organisms?
They form covalent bonds rather than becoming ions.
What is a hydrogen bond?
A weak bond where a hydrogen atom of one molecule is attracted to an oxygen or nitrogen atom in another molecule.
What is the significance of hydrogen bonds in biological systems?
They serve as bridges between different molecules or parts of the same molecule.
What is the molecular formula for hydrogen?
H2
What is the structural formula for methane?
H-C-H with H atoms bonded to the carbon atom.
What type of bond forms between two hydrogen atoms to create a hydrogen molecule?
A single covalent bond.
What is a covalent bond?
A bond formed when two atoms share electrons.
How do hydrogen bonds differ from covalent bonds?
Hydrogen bonds are considerably weaker than covalent bonds, having only about 5% of their strength.
What is the molecular mass of water (H2O)?
18 daltons (Da) or atomic mass units (amu).
What is one mole of a substance?
Its molecular mass expressed in grams.
Differentiate an ionic bond from a covalent bond.
An ionic bond is formed by the transfer of electrons, while a covalent bond is formed by the sharing of electrons.
What is an ionic bond?
An attraction between ions of opposite charge that holds them together to form a stable molecule.
What is a covalent bond?
A bond formed by two atoms that share one or more pairs of electrons, and is the most common type of chemical bond in organisms.
What is a hydrogen bond?
A relatively weak bond where a hydrogen atom covalently bonded to one oxygen or nitrogen atom is attracted to another oxygen or nitrogen atom.
What are anabolic reactions?
Pathways of synthesis reactions in living organisms, such as combining sugar molecules to form starch and amino acids to form proteins.
What is a decomposition reaction?
The reverse of a synthesis reaction, where bonds are broken and large molecules are split into smaller molecules, ions, or atoms.
What are catabolic reactions?
Decomposition reactions that occur in living organisms, such as the breakdown of sucrose into glucose and fructose during digestion.
What is activation energy?
The initial energy needed to break a bond in a chemical reaction.
What is an endergonic reaction?
A chemical reaction that absorbs more energy than it releases, directing energy inward.
What is an exergonic reaction?
A chemical reaction that releases more energy than it absorbs, directing energy outward.
What is an exchange reaction?
A reaction that is part synthesis and part decomposition, where reactants recombine to form new products.
What is the general formula for a synthesis reaction?
Reactants combine to form products, expressed as A + B → AB.
What is the general formula for a decomposition reaction?
Reactant breaks down into products, expressed as AB → A + B.
What is the significance of chemical reactions in metabolism?
Chemical reactions involve the making or breaking of bonds, allowing cells to do work by releasing energy when new bonds are formed.
What does it mean for a chemical reaction to be reversible?
It means that the reaction can occur in either direction, although some reactions reverse more easily than others.
What is a reversible reaction in chemistry?
A reversible reaction is one where the end product can revert to the original molecules, indicated by two arrows.
What are the two principal classes of compounds in biology and chemistry?
Inorganic and organic compounds.
What is the pH of a neutral solution?
7, where the concentrations of H+ and OH- are equal.
What defines inorganic compounds?
Inorganic compounds are usually small, structurally simple molecules that typically lack carbon and may involve ionic bonds.
What defines organic compounds?
Organic compounds always contain carbon and hydrogen and are typically structurally complex.
What is the role of water in living organisms?
Water is vital for growth, repair, maintenance, and reproduction, and it serves as a medium for most chemical reactions inside cells.
What percentage of every cell is made up of water on average?
Water makes up 65-75% of every cell on average.
What is a polar molecule?
A polar molecule is one that has an unequal distribution of charges, such as water, which has a slightly negative charge on the oxygen region and a slightly positive charge on the hydrogen region.
How many hydrogen bonds can each water molecule form?
Each water molecule can form four hydrogen bonds with nearby water molecules.
Why does ice float on water?
Ice is less dense than liquid water due to the crystalline structure formed by hydrogen bonds, which makes it take up more space.
What is the significance of water's high boiling point?
Water's high boiling point allows it to exist in the liquid state on most of the Earth's surface, which is essential for life.
What happens during the ionization of sodium chloride (NaCl) in water?
Sodium chloride dissociates into sodium ions (Na+) and chloride ions (Cl-) due to the polarity of water, which disrupts the ionic bonds between them.
How does water act as a solvent for sodium chloride?
Water molecules surround the sodium and chloride ions, with the oxygen atoms attracted to the sodium ion and the hydrogen atoms attracted to the chloride ion, facilitating the dissolution process.
What defines an acid in terms of ionization?
An acid is a substance that dissociates into one or more hydrogen ions (H+) and one or more negative ions (anions).
What defines a base in terms of ionization?
A base dissociates into one or more negatively charged hydroxide ions (OH-) and one or more positive ions (cations).
What is the role of water in biochemical reactions?
Water acts as a reactant or product in many chemical reactions, facilitating the breakdown of larger molecules and the synthesis of organic compounds.
What is the significance of maintaining acid-base balance in organisms?
Maintaining a constant balance of acids and bases is crucial for enzyme function and overall cellular health, as deviations can disrupt biochemical processes.
What does the pH scale represent?
The pH scale expresses the concentration of hydrogen ions (H+) in a solution, ranging from 0 to 14, where lower values indicate higher acidity and higher values indicate higher alkalinity.
How do acids and bases differ in their dissociation in water?
Acids dissociate to release H+ ions, while bases dissociate to release OH- ions; salts dissociate into cations and anions that are neither H+ nor OH-.
What is the effect of a change in pH on biochemical reactions?
Even small changes in pH can dramatically affect biochemical reactions, as they are sensitive to the concentrations of H+ and OH- ions.
How can the pH of a solution be changed?
By adding substances that increase the concentration of hydrogen ions, thus increasing its acidity.
What role do pH buffers play in living organisms?
They help keep the pH from changing drastically, maintaining a stable environment for biochemical reactions.
What is the optimal pH range for most organisms?
Between 6.5 and 8.5.
Which type of microbes can tolerate acidic conditions best?
Fungi.
What is the natural environment pH of Cutibacterium acnes?
About 4, which is slightly acidic.
What is the pH range for optimum growth of Acidithiobacillus ferrooxidans?
From 1 to 3.5.
What happens to the pH of a laboratory medium when bacteria excrete waste products?
The pH can become acidic enough to inhibit bacterial enzymes and kill the bacteria.
What is the significance of the pH scale in relation to acidity and alkalinity?
As pH values decrease from 14 to 0, the H+ concentration increases, indicating higher acidity.
What is the difference between a polymer and a monomer?
A polymer is a large molecule made up of many repeating units called monomers, which are small molecules.
What is the role of ATP in cellular activities?
ATP is the cell's chief energy provider.
What are the main elements that make up carbohydrates?
Carbon, hydrogen, and oxygen atoms.
What is the general formula for carbohydrates?
(CH₂O)ₙ where n indicates that there are three or more CH₂O units.
What are the three major groups of carbohydrates based on size?
Monosaccharides, disaccharides, and polysaccharides.
What are monosaccharides?
Simple sugars that contain three to seven carbon atoms.
What is an example of a pentose sugar?
Deoxyribose, which is found in DNA.
What is glucose?
A common hexose and the main energy-supplying molecule of living cells.
How are disaccharides formed?
When two monosaccharides bond in a dehydration synthesis reaction.
What is sucrose?
A disaccharide formed from glucose and fructose.
What is hydrolysis?
The chemical reaction that breaks down disaccharides into simpler molecules by adding water.
What are polysaccharides?
Carbohydrates consisting of tens or hundreds of monosaccharides joined through dehydration synthesis.
What is glycogen?
A polysaccharide composed of glucose subunits, synthesized as a storage material by animals and some bacteria.
What is cellulose?
A glucose polymer that is the main component of the cell walls of plants and most algae.
What is chitin?
A polysaccharide that makes up part of the cell wall of most fungi and the exoskeletons of lobsters, crabs, and insects.
What role do lipids play in living cells?
They are essential to the structure and function of membranes that separate living cells from their environment.
What is a common characteristic of lipids?
They are nonpolar molecules and are insoluble in water.
What are the two structural forms of starch found in plants?
Amylopectin and amylose.
Which type of starch is rapidly converted into glucose in the small intestine?
Amylopectin.
Why is amylose more resistant to digestion compared to amylopectin?
Amylose has less surface area for enzymes to react with, making it more resistant to digestion.
What health benefits are associated with amylose-fermenting bacteria?
They produce short-chain fatty acids that are important for intestinal health and may protect against colorectal cancer.
What is butyrate and its significance in relation to Prevotella metabolism?
Butyrate is a type of short-chain fatty acid linked to Prevotella metabolism, which may protect against colorectal cancer.
What was the outcome of the study involving mice treated with antibiotics and Clostridioides difficile?
Mice with large numbers of Lachnospira bacteria were more likely to survive, while those dominated by Escherichia coli were more likely to die.
What are simple lipids commonly known as?
Fats.
What are triglycerides composed of?
An alcohol called glycerol bonded to three fatty acids.
What is the primary function of lipids in living organisms?
To form plasma membranes that enclose cells.
What determines whether a fat molecule is a monoglyceride, diglyceride, or triglyceride?
The number of fatty acid molecules combined with glycerol.
What is the difference between saturated and unsaturated fatty acids?
Saturated fatty acids have no double bonds and contain the maximum number of hydrogen atoms, while unsaturated fatty acids have one or more double bonds.
What are the components that form a triglyceride molecule?
One molecule of glycerol and three fatty acid molecules.
What is the difference between saturated and unsaturated fatty acids?
Saturated fatty acids are relatively straight and can pack closely together, while unsaturated fatty acids have kinks due to double bonds, preventing close packing.
What is a cis fatty acid?
An unsaturated fatty acid where the hydrogen atoms on either side of the double bond are on the same side of the chain.
What is a trans fatty acid?
An unsaturated fatty acid where the hydrogen atoms on either side of the double bond are on opposite sides of the chain.
What are phospholipids made of?
Glycerol, two fatty acids, and a phosphate group bonded to an organic group.
What role do phospholipids play in cells?
They build membranes and are essential for a cell's survival.
What is the significance of the polar and nonpolar regions in phospholipids?
The polar regions orient towards water, forming hydrogen bonds, while nonpolar regions interact with neighboring nonpolar molecules, creating a barrier for cell membranes.
How do phospholipids contribute to the structure of plasma membranes?
They form a bilayer that separates the cell's contents from the water-based environment.
What complex lipids are found in the cell wall of Mycobacterium tuberculosis?
Waxes and glycolipids.
Where are phospholipids found in cells?
In the plasma membrane of cells.
What are steroids structurally different from?
Lipids.
What is the structure characteristic of steroids?
Four interconnected carbon rings.
What is a sterol?
A steroid with an -OH group attached to one of the rings.
What is an example of a sterol found in fungi?
Ergosterol.
What role do sterols play in cells?
They are important constituents of the plasma membranes of animal cells and some bacteria, fungi, and plants.
What distinguishes one amino acid from another?
The side group (R group) attached to the alpha-carbon.
What are the building blocks of proteins?
Amino acids.
What is the general structure of an amino acid?
An amino group (-NH2), a carboxyl group (-COOH), and a side group (R) attached to a central carbon atom (C).
What type of amino acids are most metabolically important?
Alpha-amino acids.
What are the two configurations of amino acids called?
Stereoisomers, designated by D and L.
Which isomer of amino acids is always found in proteins?
L-amino acids.
What role do proteins play in cells?
They are essential for cell structure and function, including acting as enzymes, transporters, and structural components.
What are some functions of proteins in biological systems?
Enzymes speed up biochemical reactions, transporter proteins move chemicals, and some proteins have regulatory functions.
What is the significance of the side groups in amino acids?
They affect the total structure and function of proteins.
What are the 20 amino acids found in proteins?
Glycine, Serine, Aspartic acid, Phenylalanine, Alanine, Threonine, Lysine, Tyrosine, Valine, Cysteine, Arginine, Histidine, Leucine, Methionine, Asparagine, Tryptophan, Isoleucine, Glutamic acid, Glutamine, Proline.
Which amino acids contain sulfur?
Cysteine and Methionine.
What is the significance of the R group in amino acids?
The R group determines the characteristics and properties of each amino acid.
What type of group does Lysine contain?
An additional amino (-NH2) group, making it basic.
What type of group does Aspartic acid contain?
An additional carboxyl (-COOH) group, making it acidic.
What is unique about Proline among the amino acids?
Proline is a heterocyclic amino acid.
Which amino acid is known for having a cyclic structure?
Phenylalanine and Tyrosine have cyclic structures.
What is the role of Methionine in amino acids?
Methionine contains a thioether (SC) group.
Which amino acids are classified as branched chain amino acids?
Valine, Leucine, and Isoleucine are branched chain amino acids.
What is the structure of Tryptophan?
Tryptophan has a heterocyclic structure.
What is the source of Jonathan's B. anthracis infection?
Environmental investigations uncovered B. anthracis at Jonathan's home, in his van, and in his workplace.
Why are B. anthracis capsules resistant to digestion by phagocytes?
B. anthracis cells have capsules composed of poly-D-glutamic acid, which helps them evade phagocytosis.
What are peptide bonds?
Peptide bonds are the bonds formed between the carbon atom of the carboxyl group of one amino acid and the nitrogen atom of the amino group of another amino acid.
What is dehydration synthesis in the context of peptide bond formation?
Dehydration synthesis is the process where one water molecule is released for every peptide bond formed between two amino acids.
What is the primary structure of a protein?
The primary structure is the unique sequence in which the amino acids are linked together to form a polypeptide chain.
What is the secondary structure of a protein?
The secondary structure is the localized, repetitious twisting or folding of the polypeptide chain, resulting from hydrogen bonds.
What does tertiary structure refer to in proteins?
Tertiary structure refers to the overall three-dimensional structure of a polypeptide chain, involving interactions between various amino acid side groups.
How are amino acids related to proteins?
Amino acids are the building blocks of proteins, linked together by peptide bonds to form polypeptide chains.
What is the primary structure of a protein?
The primary structure is the polypeptide strand, which is the amino acid sequence.
What stabilizes the secondary structure of proteins?
Hydrogen bonds between the amino acids stabilize the secondary structure, which includes helix and pleated sheet formations.
What is the tertiary structure of a protein?
The tertiary structure is the overall three-dimensional folding of a polypeptide chain, which includes interactions like hydrophobic interactions and disulfide bridges.
What role do disulfide bridges play in protein structure?
Disulfide bridges are strong covalent bonds that help stabilize the protein structure by linking cysteine molecules together.
What is the quaternary structure of a protein?
The quaternary structure consists of an aggregation of two or more individual polypeptide chains (subunits) that operate as a single functional unit.
What can cause a protein to undergo denaturation?
Denaturation can occur due to hostile environments such as extreme temperature, pH, or salt concentrations, leading to the loss of its characteristic shape.
What are some examples of proteins with quaternary structure?
Examples include hemoglobin, microfilaments, microtubules, the virus coat, and DNA polymerase.
How are the two strands of DNA oriented in relation to each other?
They are antiparallel, meaning one strand runs in the opposite direction to the other.
What forms the backbone of the DNA double helix?
Alternating sugar and phosphate groups.
Which nitrogenous bases pair together in DNA?
Adenine pairs with Thymine, and Guanine pairs with Cytosine.
What is the significance of understanding DNA's structure and function?
It is essential for understanding genetics, recombinant DNA techniques, and the emergence of antibiotic resistance and new diseases.
What type of bonds hold the complementary base pairs together in DNA?
Hydrogen bonds.
What is the structure of DNA described as?
A double-helical, ladderlike form.
What is the role of the sugar-phosphate backbone in DNA?
It provides structural support and stability to the DNA molecule.
How do DNA and RNA differ in structure?
DNA is double-stranded while RNA is usually single-stranded; RNA contains ribose sugar instead of deoxyribose, and RNA has uracil (U) instead of thymine.
What are the three major kinds of RNA identified in cells?
Messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA).
What is the principal energy-carrying molecule of all cells?
Adenosine triphosphate (ATP).
What happens when the third phosphate group of ATP is hydrolyzed?
It releases usable energy, converting ATP into adenosine diphosphate (ADP) and inorganic phosphate.
How is ATP produced in cells?
By adding a phosphate group to ADP using energy supplied by the cell's oxidation reactions, particularly the oxidation of glucose.
What is the structure of ATP?
ATP consists of an adenosine unit (adenine and ribose) with three phosphate groups attached.
What is the significance of ATP in cellular processes?
ATP stores chemical energy released by reactions and provides energy for reactions that require energy, making it indispensable to cell survival.
How are ATP and RNA nucleotides similar?
Both ATP and RNA nucleotides contain ribose sugar and a nitrogenous base (adenine in ATP and uracil or other bases in RNA).
What is the backbone sugar in DNA?
Deoxyribose.
What are the nitrogen-containing bases found in DNA?
Cytosine (C), guanine (G), adenine (A), and thymine (T).
What is the backbone sugar in RNA?
Ribose.
What are the nitrogen-containing bases found in RNA?
Cytosine (C), guanine (G), adenine (A), and uracil (U).
What is the primary function of DNA?
Protein synthesis and determining all hereditary traits.
What is one of the functions of RNA?
Protein synthesis, genetic code of some viruses, and gene deactivation.
What is an atom?
The smallest unit of a chemical element that exhibits the properties of that element.
What are the components of an atom?
A nucleus containing protons and neutrons, and electrons that move around the nucleus.
What is the atomic number?
The number of protons in the nucleus of an atom.
What are isotopes?
Atoms that have the same atomic number but different atomic masses.
What determines the chemical properties of an atom?
The number of electrons in its outermost (valence) shell.
What are molecules made up of?
Two or more atoms; molecules with at least two different kinds of atoms are called compounds.
What is a chemical bond?
Attractive forces that bind two atoms together.
What are functional groups responsible for?
Most of the properties of organic molecules.
What do the letters R represent in organic chemistry?
The remainder of an organic molecule.
What are two frequently encountered classes of organic molecules?
R-OH (alcohols) and R-COOH (organic acids).
What are macromolecules?
Very large molecules formed from small organic molecules.
How do monomers bond together?
By dehydration synthesis, forming water and a polymer.
What is hydrolysis?
A reaction involving the splitting of water molecules to break down organic molecules.
What are carbohydrates composed of?
Atoms of carbon, hydrogen, and oxygen, with hydrogen and oxygen in a 2:1 ratio.
What do monosaccharides contain?
From three to seven carbon atoms.
What are isomers?
Two molecules with the same chemical formula but different structures and properties.
How do monosaccharides form disaccharides and polysaccharides?
By dehydration synthesis.
What distinguishes lipids from other compounds?
Their insolubility in water.
What do simple lipids consist of?
A molecule of glycerol and three molecules of fatty acids.
What is the difference between saturated and unsaturated lipids?
Saturated lipids have no double bonds between carbon atoms; unsaturated lipids have one or more double bonds.
What are phospholipids composed of?
Glycerol, two fatty acids, and a phosphate group.
What are steroids characterized by?
Four interconnected carbon ring structures.
What are amino acids?
The building blocks of proteins.
What elements do amino acids consist of?
Carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.
How many amino acids occur naturally in proteins?
Twenty amino acids.
What type of bonds allow the formation of polypeptide chains?
Peptide bonds formed by dehydration synthesis.
What are the four levels of protein structure?
Primary, secondary, tertiary, and quaternary.
What are conjugated proteins?
Proteins combined with inorganic or other organic compounds.
What are nucleic acids?
Macromolecules consisting of repeating nucleotides.
What is a nucleotide composed of?
A pentose, a phosphate group, and a nitrogen-containing base.
What is the structure of a DNA nucleotide?
It consists of deoxyribose, a phosphate group, and one of the nitrogen-containing bases: thymine, cytosine, adenine, or guanine.
What is the structure of DNA?
Two strands of nucleotides wound in a double helix held together by hydrogen bonds.
What do genes consist of?
Sequences of nucleotides.
What is an RNA nucleotide composed of?
Ribose, a phosphate group, and one of the nitrogen-containing bases: cytosine, guanine, adenine, or uracil.
What is the function of ATP?
It stores chemical energy for various cellular activities.
How is ATP regenerated from ADP?
The energy from oxidation reactions is used to regenerate ATP from ADP and inorganic phosphate.
What is a chemical element?
A substance that cannot be broken down into simpler substances by chemical means.
What type of bond holds Li+ and Cl- in LiCl?
Ionic bond.
What type of bond holds carbon and oxygen atoms in methanol?
Covalent bond.
What type of bond holds oxygen atoms in O₂?
Covalent bond.
What type of bond holds a hydrogen atom of one nucleotide to a nitrogen or oxygen atom of another nucleotide?
Hydrogen bond.
Classify the reaction: glucose + fructose → sucrose + H₂O.
Synthesis reaction.
Classify the reaction: lactose → glucose + galactose.
Decomposition reaction.
Classify the reaction: NH₄Cl + H₂O → NH₄OH + HCl.
Exchange reaction.
Classify the reaction: ATP → ADP + Pi.
Decomposition reaction.
What purpose does the enzyme urease serve in the reaction CO(NH₂)₂ + H₂O → 2NH₃ + CO₂?
It catalyzes the reaction, speeding up the conversion of urea to ammonia and carbon dioxide.
What type of subunit is oleic acid?
Lipid.
What type of subunit is serine?
Protein.
What type of subunit is C₆H₁₂O₆?
Carbohydrate.
What type of subunit is thymine nucleotide?
Nucleic acid.
What types of molecules are aspartic acid and phenylalanine?
Amino acids.
What direction is the hydrolysis reaction in the synthesis of aspartame?
Right to left.
What direction is the dehydration synthesis reaction in the synthesis of aspartame?
Left to right.
What type of microorganism has a chitin cell wall, has DNA contained in a nucleus, and has ergosterol in its plasma membrane?
Fungi.
What is the most likely location of 16 N in E. coli bacteria after 48 hours in a nutrient medium containing it?
In the carbohydrates, lipids, proteins, or water of E. coli bacteria.
If Pseudomonas bacteria are supplied with radioactively labeled cytosine, where would this cytosine most likely be found after 24 hours?
In the DNA of the cells.
In which molecules would 32 P not be found in E. coli grown in a medium containing it?
In carbohydrates.
How many times more acidic is the optimum pH of Acidithiobacillus bacteria compared to blood?
10,000 times more acidic.
What is the best definition of ATP?
A molecule that supplies energy to do work.
Which of the following is an organic molecule?
C₁₈H₂₉SO₃ (Styrofoam).
What type of reaction is A in the equation H₂O + CO₂ ᴬH₂CO₃?
A synthesis reaction.
What does reaction B in the equation H₂CO₃ ᴮH⁺ + HCO₃⁻ tell you about H₂CO₃?
It indicates that H₂CO₃ is an acid.
What happens to the relative amount of unsaturated lipids in the plasma membrane when E. coli bacteria are grown at 37°C compared to 25°C?
The relative amount of unsaturated lipids decreases.
How do giraffes, termites, and koalas obtain nutrition from plant matter despite being unable to digest cellulose?
They rely on symbiotic bacteria or enzymes that can break down cellulose.
What type of molecule is poly-3-hydroxybutyrate (PHB) and why would a cell store it?
PHB is a polymer and cells store it as an energy reserve.
How does Acidithiobacillus ferrooxidans cause the change from lime to gypsum?
By metabolizing pyrite and producing sulfuric acid, which reacts with lime to form gypsum.
What type of chemical is phenylalanine?
Phenylalanine is an amino acid.
What does 'no growth' in the Guthrie test for PKU indicate?
It indicates that the baby does not have PKU and can metabolize phenylalanine.
Why must individuals with PKU avoid the sweetener aspartame?
Because aspartame contains phenylalanine, which they cannot metabolize.
Would you expect to use amphotericin B against a bacterial infection? A fungal infection?
Amphotericin B is used against fungal infections, not bacterial infections, because it targets sterols in fungal membranes.
What amino acids are expected in eggs that produce a sulfur smell when boiled?
Cysteine and methionine, which contain sulfur.
What is the purpose of a microscope in microbiology?
To observe microorganisms that are too small to be seen with the unaided eye.
What does the term 'microscope' derive from?
The Latin word 'micro' meaning small and the Greek word 'skopos' meaning to look at.
What significant advancement in microscopy occurred after 1886?
The resolving ability of microscopes improved, allowing for better observation of bacteria like Helicobacter pylori.
What is Helicobacter pylori and where does it live?
A spiral-shaped bacterium that lives in the human stomach and can cause ulcers.
Why do many microbes require staining procedures before observation?
To make their cell walls, capsules, and other structures visible, as they are often colorless in their natural state.
What is the significance of the acid-fast stain in diagnosing infections?
It helps identify certain bacteria, such as those causing tuberculosis, which are resistant to standard staining methods.
What does 'acid-fast +' indicate in a patient's lab results?
It suggests the presence of acid-fast bacteria, which may indicate an infection such as tuberculosis.
What measurement system is discussed for studying microbes?
The metric system.