What are viruses classified as?
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Acellular entities.
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What are viruses classified as?
Acellular entities.
Why are smaller free-living cells more efficient than larger ones?
Because any given mass of nutrients will support the synthesis of more small cells than large cells.
What can a single microbial cell placed on a solid nutrient medium do?
It can grow and divide into millions or even billions of cells that form a visible colony.
How many major phylogenetic lineages (phyla) of Bacteria have at least one species that has been grown in culture?
30 major phylogenetic lineages.
What percentage of cultivated bacteria belong to the four main phyla?
More than 90%.
What is systematics?
The study of the diversity of life and the relationships among living things through time, using taxonomy to understand organisms.
What is the purpose of characterization in microbiology?
For identification purposes, comparison with other organisms, and to exploit beneficial characteristics.
What was the atmospheric condition of Earth during the first 2 billion years?
Anoxic (O2 was absent).
What is the specialized chamber in the digestive tract of ruminants called?
Rumen.
How do the phylogenetic ages of Eukarya compare to Bacteria and Archaea?
Eukarya are phylogenetically relatively young compared with Bacteria and Archaea.
What is the last universal common ancestor known as?
LUCA.
What is the estimated number of microbial cells on Earth?
2 x 10^30 microbial cells.
What is the size range of Thiomargarita?
750 µm to 0.75 mm.
What is the ultimate goal of viral genome replication?
To have an mRNA+ strand.
What is the relationship between cell radius and surface area?
There is an inverse relationship: lower radius results in higher surface area.
What is a taxon?
A taxon is a group of organisms.
What are the shapes formed by cocci during cell division?
Pairs (diplococci), long chains (streptococci), three-dimensional cubes (tetrads or sarcinae), and grapelike clusters (staphylococci).
What was the common belief about the origin of life before Pasteur?
Life arose spontaneously from nonliving materials, known as spontaneous generation.
What is a strain in microbiology?
A population of organisms that descends from a pure culture isolate or from a species, which may have different attributes despite being nearly identical genetically.
Who invented the first compound microscope?
Zaccharias Janssen in 1597.
Where is DNA located in eukaryotic cells?
Within the membrane-enclosed nucleus as several linear molecules.
What did Pasteur's work on optical isomers reveal?
Living organisms could discriminate between optical isomers, suggesting many abiotic reactions were catalyzed by microbes.
What is primary endosymbiosis?
One cell must engulf another to form mitochondria and chloroplasts.
What type of genome does Class 2 viruses have?
Single-stranded (+) strand genome.
What are pure cultures?
Cultures that contain cells from only a single type of microorganism.
What is the purpose of identification in microbiology?
To characterize an isolate and determine its species, verifying authenticity and differentiating between organisms.
What did John Needham claim about organic matter?
He claimed it contained 'vile matter'.
What is taxonomy?
The science of biological classification, including describing, identifying, classifying, and naming organisms.
What is microbiology?
The study of organisms and agents too small to be seen by the naked eye, including those that can exist as single cells or acellular forms and contain a nucleic acid genome.
Who was Carolus Linnaeus?
A Swedish botanist, zoologist, and physician known for proposing the Linnaean taxonomy and publishing 'Systema Naturae'.
Why do humans rely on their gut microbiome?
To break down complex carbohydrates, as human enzymes lack this ability.
What does medical microbiology study?
Pathogenic microorganisms and their role in human illnesses, including microbial pathogenesis and infection.
What is the role of industrial microbiology?
Exploitation of microorganisms for industrial processes, such as industrial fermentation and wastewater treatment.
What is microbial ecology?
The study of the function and diversity of various groups of microorganisms.
What are the major lineages of Eukarya traditionally called?
Kingdoms instead of phyla.
What is a microbial culture?
A collection of cells that have been grown in or on a nutrient medium.
What does the Spontaneous Generation Theory propose?
Life emerges from non-living matter and requires a 'vital force'.
What distinguishes prokaryotic cells from eukaryotic cells?
Prokaryotic cells have a nucleoid instead of a nucleus and may have plasmids.
How many described phyla are there in the domain Archaea?
Five described phyla.
What does the International Code for the Nomenclature of Bacteria provide?
Rules for naming bacteria.
What are the two interconnected themes of microbiology?
Understanding the living world of microscopic organisms and applying this understanding for the benefit of humankind and the planet.
What is binomial nomenclature?
A two-word naming system for identifying organisms by genus and species.
What are microorganisms also called?
Microbes.
What properties does the Piptoporus betulinus fungus have?
Laxative and antibiotic properties.
What is a medium in microbiology?
A solid nutrient mixture that contains all of the nutrients required for a microorganism to grow.
What are the major morphologies of prokaryotic cells?
Coccus (circular), Rods/Bacillus (rod-shaped), Spirillum (curved), and Spirochete (spiral and flexible).
What is the scientific name of the yeast responsible for making bread rise?
Saccharomyces cerevisiae.
What do all cells possess?
A DNA genome, which is the full set of genes in a cell.
What structures do bacteria form on the roots of legumes?
Nodules.
What was the result of Theodore Schwann's experiment?
No growth of microorganisms was observed after passing air through red-hot tubes.
What did Robert Hooke observe with his microscope?
Cells in cork and elongated stalks of fungi.
What role do microorganisms play in relation to food?
They assist in the digestion of complex carbohydrates and contribute to human nutrition.
What is vertical gene transfer?
Gene transfer from parent to offspring.
What is Class 1 and 7 in the Baltimore classification?
Genome is double-stranded DNA.
What is the role of yeast in fermentation?
Yeast fermentation yields ethanol and CO2.
How old is Earth?
About 4.6 billion years old.
What does classification involve?
Grouping organisms into taxa based on mutual similarity or evolutionary relatedness.
What type of chromosomes do eukaryotic cells have?
Linear chromosomes.
What structures did early phototrophs live in?
Microbial mats.
What percentage of food energy do complex carbohydrates represent that humans cannot digest?
10–30%.
What specific mold did Pasteur study in relation to fermentation?
The mold Aspergillus, which metabolized exclusively D-tartrate but not L-tartrate.
What is horizontal gene transfer?
Gene transfer from different species, primarily seen in bacteria.
What does 'growth' refer to in microbiology?
The increase in cell number as a result of cell division.
What are the three major groups of cells?
Bacteria, Archaea, and Eukarya.
What are the four main phyla of cultivated bacteria?
Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes.
What was Lazzaro Spallanzani's position on Needham's conclusions?
He did not accept Needham's conclusions.
What environments have Archaea historically been associated with?
Extreme environments such as hot, salty, or acidic sites.
What are the official publications for taxonomy and classification of microorganisms?
International Journal of Systematic Bacteriology (IJSB) and International Journal of Systematic and Evolutionary Microbiology (IJSEM).
What is the significance of cell division in morphology?
Cell division impacts morphology because attached cells can form distinctive shapes.
What are the principles of nomenclature regarding generic and specific names?
The generic name can change with new information, while the specific name is stable and the oldest epithet takes precedence.
What is the focus of immunology in microbiology?
How the body reacts to infections caused by microorganisms and the antibodies produced.
How does cell size affect the surface-to-volume (S/V) ratio?
As cell size decreases, the S/V ratio increases, allowing small cells to exchange nutrients and wastes more rapidly.
What is secondary endosymbiosis?
The primary product has been engulfed again.
What is aseptic technique?
A collection of practices that allow for the preparation and maintenance of sterile nutrient media and solutions.
What is the Biogenesis Theory?
The theory that 'life begets life'.
What is the significance of Bergey's Manual?
It serves as a standard reference for identifying and classifying prokaryotes.
What type of organism is yeast?
Yeast is a eukaryotic fungus.
How are the genomes of prokaryotic and eukaryotic cells organized?
Into structures called chromosomes.
What types of microorganisms could survive in an anoxic atmosphere?
Only microorganisms capable of anaerobic metabolism.
What did supporters of spontaneous generation believe about strong acids and heat?
They believed that strong acids and heat altered the air so it could not support microbial growth.
What is the nucleoid in prokaryotic cells?
A mass of chromosome aggregates visible in the electron microscope, not enclosed by a membrane.
What are spores used for in fungi?
To multiply.
What is the size range of Epulopicium cells?
650 µm to 700 µm.
Why are viruses not considered truly alive?
They are obligate parasites that can only replicate within a host cell.
How do spirochetes differ from spirilla?
Spirochetes are flexible, while spirilla are rigid.
How is a species defined in prokaryotes?
As a collection of strains that share stable properties in common and differ significantly from other groups of strains.
What ancient civilization attributed disease to bad air?
The Ancient Greeks.
What are filamentous bacteria?
Long, thin, rod-shaped bacteria that divide terminally and form long filaments composed of many cells attached end to end.
Why are microorganisms important?
They were the first living organisms on the planet, live everywhere life is possible, are more numerous than any other kind of organism, and influence global ecosystems and human society.
What method did George Friedrich Schroder and Theodor von Dusch use in their experiments?
They filtered air through sterile cotton wool.
How many universally present genes are found in all cellular organisms?
Approximately 60 genes.
What is the difference between broth and agar?
Broth is a liquid medium used for high cell growth, while agar is a solid medium.
What are eukaryotic cells characterized by?
They are membrane-bound organelles.
What type of cell structure do Archaea have?
Prokaryotic cell structure.
What is a gene?
A segment of DNA that encodes a protein or an RNA molecule.
Do Archaea contain any known disease-causing species?
No, Archaea lack any known disease-causing species of plants or animals.
What are the most well-known groups of Eukarya?
Plants, animals, and fungi.
What is the arrangement of Dipplococcus bacteria?
Arranged in chains.
What does 'Candidatus' signify in taxonomy?
A provisional taxonomic name appended to candidate taxonomic ranks, used when there is no pure culture yet.
What role do microorganisms play in global biomass?
They constitute a major fraction and are key reservoirs of nutrients essential for life.
How many kingdoms of Eukarya are there at least?
At least six kingdoms.
What is unique about Class 6 viruses?
They are retroviruses that use reverse transcriptase to form a double-stranded intermediate.
What do enrichment culture techniques facilitate?
The isolation of microbes having particular metabolic characteristics from nature.
Who disproved the idea that maggots are spontaneously generated?
Francisco Redi in 1668.
What is a plasmid?
An extrachromosomal DNA found in some prokaryotic cells.
When did microbial cells first appear on Earth?
Between 3.8 and 4.3 billion years ago.
What experiment did Franz Schultze conduct related to spontaneous generation?
He passed air through strong acids.
What is the largest bacterium ever found?
Thiomargarita.
What are the main fields of basic microbiology?
Virology (viruses), Mycology (fungi), Phycology (algae), Protozoology (protozoa), and Bacteriology (bacteria).
How do microorganisms typically live?
In complex microbial communities.
What does agricultural microbiology study?
Agriculturally relevant microorganisms, including plant microbiology and pathology.
What does nomenclature refer to in microbiology?
The assignment of names to taxonomic groups in agreement with published rules.
What is required for the characterization and identification of microorganisms?
A pure culture.
How is a species defined in eukaryotes?
As a group of closely related organisms that breed among themselves.
What sanitation infrastructure did the Romans create?
Aqueducts and the Cloaca Maxima.
What type of chromosomes do prokaryotic cells have?
Circular chromosomes.
What percentage of life's history on Earth was exclusively microbial?
80%.
What are plasmids?
Small circles of DNA distinct from the chromosome, often found in prokaryotic cells.
What are microorganisms that thrive in harsh environments called?
Extremophiles.