Do membrane proteins move?
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Yes, membrane proteins can move within the lipid bilayer.
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Do membrane proteins move?
Yes, membrane proteins can move within the lipid bilayer.
What is the site of protein synthesis in a cell?
Ribosomes.
How do organisms living in variable temperature conditions adapt their lipid composition?
They change lipid composition in response to changing temperature.
What does resolution measure in microscopy?
The clarity of the image or the minimum distance of two distinguishable points.
How do large molecules move in and out of the cell?
Through bulk transport methods such as exocytosis or endocytosis.
What is an isotonic solution?
A solution with the same solute concentration as that inside the cell.
How can expressing thanks impact relationships?
It can strengthen bonds and foster goodwill.
What is a vacuole?
A membrane-bound organelle found in plant or fungal cells.
What is the role of the endomembrane system in a cell?
It is a complex and dynamic player in the cell's compartmental organization.
What type of hydrocarbon tails do fish in extreme cold environments have in their cell membranes?
A high proportion of unsaturated hydrocarbon tails.
What primarily holds membranes together?
Weak hydrophobic interactions.
What are thylakoids?
Membranous sacs within chloroplasts.
How do microfilaments enable movement in amoebas?
They facilitate the movement of amoebas.
What is the significance of the year 2021 in the context of the provided text?
It indicates the copyright year for the content.
What is one primary function of intermediate filaments?
To maintain the shape of the cell, including the nucleus.
What are transport proteins?
Proteins that allow passage of hydrophilic substances across the membrane.
What role do microfilaments play in amoeboid movement?
They cause the protrusion of pseudopodia in white blood cells (WBC).
What enzyme is present in peroxisomes?
Catalase.
Why do scientists use microscopes?
To visualize cells that are too small to see with the naked eye.
What is tonicity?
The ability of a surrounding solution to cause a cell to gain or lose water.
What defines a hypotonic solution?
A solution is hypotonic if the solute concentration is less than that inside the cell.
Why are vegetables like lettuce sprayed with water in supermarkets?
To maintain their crispness.
What happens to water in a hypotonic solution?
Net diffusion of water is from the surrounding solution to the inside of the cell.
How do membranes rich in unsaturated fatty acids compare to those rich in saturated fatty acids?
They are more fluid.
How do membrane proteins contribute to cell communication?
By acting as receptors for signaling molecules.
What triggers vesicle formation in receptor-mediated endocytosis?
Solute binding to receptors.
What is the significance of the © symbol in the context of educational materials?
It indicates copyright protection for the content.
What does each chromosome contain?
One DNA molecule associated with proteins, called chromatin.
What is the purpose of expressing gratitude?
To acknowledge kindness or help received.
How does the lipid composition of cell membranes adapt to environmental conditions?
It varies in many species, such as having a high proportion of unsaturated hydrocarbon tails in fish that live in extreme cold.
What is cell recognition?
A cell's ability to distinguish one type of neighboring cell from another.
What is the main focus of the study by Ovais et al. (2018)?
Multifunctional theranostic applications of biocompatible green-synthesized colloidal nanoparticles.
What role do membrane carbohydrates play in cell recognition?
They interact with the surface molecules of other cells, facilitating cell-cell recognition.
What defines a hypertonic solution?
A solution is hypertonic if the solute concentration is greater than that inside the cell.
What are the three types of intercellular junctions in animals?
Tight junctions, desmosomes, and gap junctions.
What is one factor that affects membrane fluidity?
Temperature.
What happens to water in a hypertonic solution?
Net diffusion of water is from inside the cell to the surrounding solution.
What is contrast in the context of microscopy?
Visible differences in brightness between parts of the sample.
What role do transport proteins play in cell membrane transport?
They assist in the movement of molecules across the cell membrane, either passively or actively.
When does water movement stop during osmosis?
When the solute concentration is equal on both sides.
Where are peripheral proteins located?
Bound to the surface of the membrane.
What is the centrosome made up of?
A pair of centrioles.
What do integral proteins do?
They penetrate the hydrophobic core of the membrane.
What is pinocytosis?
A process where molecules are taken up when extracellular fluid is 'gulped' into tiny vesicles.
What are the microfibrils in the primary cell wall made of?
Cellulose.
What is osmoregulation?
The control of solute concentration and water balance.
How many vacuoles can a plant or fungal cell have?
One or several vacuoles.
What do coated pits form during receptor-mediated endocytosis?
Coated vesicles.
What are integral proteins?
Proteins that penetrate the hydrophobic core of the lipid bilayer.
What are plastids?
Organelles found only in plants and algae.
What material is found in the cell walls of plants and algae?
Cellulose.
What is the composition of fungal cell walls?
Chitin.
What is essential for successful learning?
Communication between brain cells.
What are plasmodesmata?
Channels that perforate plant cell walls.
What structure do phospholipids form in a cell membrane?
A bilayer with hydrophobic tails inside and hydrophilic heads exposed to water.
What is the diameter of a microtubule?
0.25 μm.
What are membrane proteins?
Proteins that are part of, or interact with, cell membranes.
What technological advancement made the study of cells possible?
Microscopes.
What do plants use to buffer membrane fluidity?
Plants use different but related steroid lipids.
What is the term for the movement of molecules from high to low concentration?
Down the concentration gradient.
What happens if the surrounding solution has a higher concentration of solutes than the inside of the cell?
Water will tend to leave the cell.
How does spraying water affect the cells in vegetables?
It helps to keep the cells turgid by maintaining water pressure.
What occurs to cells without cell walls in a hypotonic solution?
They will gain water, swell, and lyse (burst).
What role do desmosomes play in animal cells?
They provide mechanical strength by anchoring adjacent cells together.
What is the primary function of the cell?
To serve as the basic unit of life.
What is the purpose of gap junctions?
To allow communication and the passage of ions and small molecules between adjacent cells.
What happens to vegetables when they lose water?
They become wilted and lose their crispness.
What are the four types of prokaryotes based on their energy and carbon sources?
Photoautotroph, chemoautotroph, photoheterotroph, and chemoheterotroph.
What can be analyzed after cell fractionation?
The structure and function of isolated organelles.
What is the significance of membrane proteins in maintaining cell shape?
They provide structural support and stability.
From where do all cells arise according to Cell Theory?
From preexisting cells.
How do microfilaments contribute to endocytosis and exocytosis?
They are involved in the invagination and evagination of the membrane.
What are peripheral proteins?
Appendages loosely bound to the surface of the membrane.
What is active transport?
Movement of small molecules that requires energy and a transport protein.
What allows membrane proteins to move?
The fluid nature of the lipid bilayer permits movement.
What effect does cholesterol have on membrane fluidity at cool temperatures?
At cool temperatures, cholesterol maintains fluidity by preventing tight packing.
What are microtubules?
Cylindrical structures that are part of the cytoskeleton in eukaryotic cells.
What is the structure of the Golgi apparatus?
It consists of flattened membranous sacs called cisternae.
What factors affect cellular processes?
Temperature, oxygen concentration ([O2]), amount of mitochondria, surface area, and inhibitors.
Which types of organisms have cell walls?
Plants, prokaryotes, fungi, and some protists.
What is the significance of membrane fluidity?
It allows for the movement of lipids and proteins within the membrane.
How are the components of the endomembrane system connected?
They are either continuous or connected via transfer by vesicles.
What is the first factor affecting diffusion?
Concentration gradient.
What is the effect of staining on Brightfield microscopy?
Staining enhances contrast, but most procedures require that cells be fixed (preserved).
What gradient does passive transport follow?
The concentration gradient.
What happens to a plant cell in an isotonic solution?
There is no net movement of water into the cell.
What sets upper limits on the size of cells?
Metabolic requirements.
What are the two types of electron microscopes?
Transmission electron microscope (TEM) and scanning electron microscope (SEM).
What is a defining feature of eukaryotic cells?
They have DNA in a nucleus that is bounded by a double membrane.
What is the healthy state for most plant cells?
Turgid (very firm).
Do plant cells have centrioles?
No, they have microtubule organizing centers that function similarly.
What role do intermediate filaments play in specialized cells?
They help some specialized cells perform their functions, such as nail-producing cells forming nails.
What tools do biologists use to study cells?
Microscopes and the tools of biochemistry.
How does cholesterol affect membrane fluidity at warm temperatures?
At warm temperatures (such as 37ºC), cholesterol restrains the movement of phospholipids.
What happens to membranes as temperatures cool?
They switch from a fluid state to a solid state.
What is the diameter of a microvillus?
0.25 μm.
What happens to the percentage of unsaturated phospholipids in winter wheat during autumn?
It increases to prevent membrane solidification during winter.
What structure is associated with the plasma membrane?
Microvillus.
What protection does the cell wall offer to plant cells?
It protects them from rupturing due to excess water movement.
What is the primary use of microscopes?
To visualize cells.
How does the presence of unsaturated fatty acids influence membrane fluidity?
Unsaturated fatty acids increase membrane fluidity.
What effect does a hypertonic solution have on cells without cell walls?
They will lose water, shrivel, and likely die.
In which type of cells are intermediate filaments found?
Only in animal cells.
Why must membranes be fluid?
To work properly.
What aids in the intake of digested food (glucose) into epithelial cells of the small intestine?
The proton pump.
What happens during phagocytosis involving lysosomes?
The food vacuole fuses with the lysosome, and hydrolytic enzymes digest food particles.
What role do lenses play in a light microscope?
Lenses refract (bend) the light to magnify the image.
What is the function of the sodium-potassium pump in nerve cells?
It maintains the potential difference between the inside and outside of nerve cells.
What is the function of the plasma membrane?
It is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste.
What do lysosomes contain that aids in digestion?
Active hydrolytic enzymes.
What type of organelles do eukaryotic cells contain?
Membrane-bound organelles.
What is the size of a lysosome?
Approximately 1 μm.
What does cryo-electron microscopy (cryo-EM) allow?
Preservation of specimens at very low temperatures.
What is active transport?
Transport of substances across a membrane against their concentration gradient.
What is one function of microfilaments during cell division?
They enable the formation of the cleavage furrow.
How do intermediate filaments support organelles?
They distribute and support organelles in the cytoplasm.
What are the two types of cell walls?
Primary cell wall and secondary cell wall.
What is the role of cholesterol in animal cell membranes?
Cholesterol is a membrane component that affects membrane fluidity at different temperatures.
What is passive transport?
Movement of small molecules across the cell membrane without energy input, possibly requiring transport proteins.
Why must membranes be fluid?
Fluidity affects both permeability and movement of transport proteins.
What is magnification in microscopy?
The ratio of an object's image size to its real size.
What is the role of the cell wall in plant cells?
It maintains the shape of plant cells.
What happens if membranes are too fluid?
They cannot support protein function.
Who identified cell walls and named them 'cells'?
Robert Hooke.
What is an electrogenic pump?
A transport protein that generates voltage across a membrane, storing energy for cellular work.
What type of nanoparticles are discussed in the study?
Biocompatible green-synthesized colloidal nanoparticles.
What is the size comparison of intermediate filaments to microfilaments and microtubules?
Bigger than microfilaments, smaller than microtubules.
In which direction do molecules move during diffusion?
From high to low concentration.
What is the secondary cell wall?
A harder and usually thicker layer of the cell wall.
What does tonicity depend on?
The concentration of solutes in the solution that cannot cross the membrane, relative to that inside the cell.
What energy source is required for bulk transport?
ATP.
What substance do peroxisomes detoxify in liver cells?
Alcohol.
What is the main electrogenic pump in animals?
The sodium-potassium pump.
What is cell fractionation?
A research method used to separate cellular components.
Where is the secondary cell wall located?
Between the plasma membrane and the primary cell wall.
Does passive transport require ATP?
No, it does not need ATP.
What is cell fractionation?
The process of taking cells apart and separating the major organelles from one another.
How do mineral ions enter root hair cells?
Through the uptake mechanisms in young epidermal cells of roots.
What is the approximate size of a peroxisome?
1 μm.
What happens to a plant cell in a hypotonic solution?
It takes up water and swells until turgor pressure is exerted.
What happens to water movement in an isotonic solution?
Water diffuses across the membrane at the same rate in both directions, resulting in no net movement.
What is the significance of unsaturated hydrocarbon tails in cell membranes?
They help maintain membrane fluidity in cold environments.
What is the primary function of the endomembrane system?
It regulates protein traffic and performs metabolic functions.
What is osmosis?
The diffusion of free water across a selectively permeable membrane.
What triggers the change in shape of membrane proteins?
Molecules released from vesicles binding to them.
What is endocytosis?
A process where macromolecules are taken into the cell in vesicles.
What is the function of centrioles?
To organize spindle fibers.
How do organisms living in extreme temperatures adapt their membranes?
They have differences in membrane lipid composition.
How does the membrane behave during endocytosis?
It forms a pocket that deepens and pinches off to form a vesicle.
What is the function of tight junctions?
To prevent the passage of materials between cells.
What is the function of membrane proteins?
They facilitate various functions such as transport, signaling, and cell recognition.
What do electron microscopes (EMs) focus to examine specimens?
A beam of electrons.
How are the microfibrils arranged in the secondary cell wall?
Regularly arranged in parallel rows, perpendicular to those in upper or lower layers.
What problems do cells without walls face in hypotonic or hypertonic environments?
They cannot tolerate excessive water loss or uptake.
What type of molecules does facilitated diffusion primarily transport?
H2O soluble molecules and molecules not soluble in lipids, such as ions, nucleic acids, amino acids, and glucose.
What is turgor pressure?
The pressure of the cell contents against the cell wall, which keeps the plant firm and crisp.
What role do fibers of the extracellular matrix play?
They provide structural support and help in cell adhesion and communication.
What are the two main types of cells?
Prokaryotic and Eukaryotic cells.
Is pinocytosis specific or nonspecific for the substances it transports?
Nonspecific; it takes in any and all solutes.
What role do microtubules play during cell division?
They help separate the chromosome copies in dividing cells.
Give an example of intermediate filaments.
Keratin filaments in hairs and nails.
What happens to the food vacuole after it is formed in phagocytosis?
It fuses with a lysosome to digest the particle.
What is the term used to describe the combined forces driving ion diffusion?
Electrochemical gradient.
What is the function of a centrosome?
It serves as the main microtubule organizing center in cells.
What type of membrane do plastids have?
Double membrane.
What movement do microtubules facilitate in cilia and flagella?
They can contract, causing movement.
What role does the rough ER play in the cell?
It acts as a membrane factory for the cell.
How do photoheterotrophs acquire energy and carbon?
They get energy from light and receive carbon by ingesting other organisms.
Can active transport occur even when there are many substances inside the cell?
Yes, more substances can still be taken in.
What is an example of a molecule that undergoes facilitated diffusion in red blood cells?
Glucose.
What is the space between the inner and outer membranes of mitochondria called?
Intermembrane space.
What do vesicles release when they fuse with the plasma membrane?
Molecules that bind to membrane proteins.
What is diffusion?
The tendency for molecules of any substance to spread out evenly into the available space.
What is bulk transport across the plasma membrane?
The movement of large molecules like proteins and polysaccharides via vesicles.
How does the cell wall contribute to plant cell structure?
It provides mechanical strength and support.
What structures do plasmodesmata connect in plant cells?
Plasma membranes and cell walls.
What determines the temperature at which a membrane solidifies?
The types of lipids present in the membrane.
What is a lysosome?
A membranous sac of hydrolytic enzymes that can digest all kinds of macromolecules.
What are microfilaments?
Thin, thread-like structures that are also part of the cytoskeleton.
What are the discrete units of DNA organized in the nucleus called?
Chromosomes.
What type of filaments are microfilaments?
Actin filaments.
What process do lysosomes use to carry out intracellular digestion?
Phagocytosis.
What is the structure of the primary cell wall?
It consists of thin layers found just outside of the plasma membrane.
What is exocytosis?
The egestion or secretion of substances from the cell.
What role do glycoproteins play in cell recognition?
They serve as identification tags recognized by other cells.
What are intermediate filaments made up of?
4 long strands of α helix coiled fibrous proteins.
What is the diameter of microtubules?
25 nm.
What does it mean for phospholipids to be amphipathic?
They contain both hydrophobic (water-fearing) and hydrophilic (water-loving) regions.
What is one benefit of cell fractionation?
It enables scientists to determine the functions of organelles.
How does a transmission electron microscope (TEM) work?
It focuses a beam of electrons through a specimen.
What is the charge of the inside of the cell relative to the outside?
Negative.
How does the size of the diffusing molecule influence diffusion?
Smaller molecules diffuse more easily and quickly.
In which direction does facilitated diffusion occur?
Down the concentration gradient.
What is simple diffusion?
The movement of molecules from an area of higher concentration to an area of lower concentration.
How does the cytoskeleton affect the shape of cells?
It determines the 3D shape of the cells.
What is the effect of polarity on the diffusion of molecules?
Non-polar molecules diffuse more easily than polar molecules.
How are cilia and flagella structured?
They consist of 9 pairs of microtubules and 2 singlet microtubules.
What is a key advantage of using cryo-EM?
It allows visualization of structures in their cellular environment without the need for preservatives.
What are the three types of plastids?
Chloroplasts, Leucoplasts, and Chromoplasts.
What type of arms are found on the outer microtubule doublets?
Dynein arms.
What are the main transport mechanisms across cell membranes?
Passive transport, active transport, and facilitated diffusion.
What is the diameter of a basal body?
0.5 μm.
What is the sodium-potassium pump?
A type of active transport that moves sodium out of the cell and potassium into the cell.
How thick are tight junctions?
0.5 μm to 1 μm.
What is the effect of an isotonic solution on a cell without a cell wall?
The volume of the cell remains stable.
What is the size of plasmodesmata?
Approximately 0.5 μm.
How often do lipids flip-flop across the membrane?
Rarely.
What are the main components of the endomembrane system?
Nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and plasma membrane.
What is the significance of membrane protein movement?
It is crucial for various cellular functions, including signaling and transport.
What is the role of the plasma membrane surrounding each cell?
Regulates exchanges with its environment and surrounding cells.
What is the diameter range of intermediate filaments?
8 - 12 nm.
Where is the primary cell wall found?
In young cells and cells that are not highly differentiated, such as meristem, parenchyma, and collenchyma.
What is endocytosis?
The uptake of substances into the cell.
What is Robert Hooke known for in cell biology?
Identifying cell walls and naming them 'cells'.
What is one function of microtubules related to cell structure?
They shape the cell by forming the cytoskeleton.
How do centrifuges contribute to cell fractionation?
They fractionate cells into their component parts through differential centrifugation.
What is phagocytosis?
A process where a cell engulfs a particle by extending pseudopodia around it.
What role does the centrosome play in animal cells?
It is considered a microtubule-organizing center.
What does the number sequence 1 2 3 5 6a 6 11 8 7 9 10 13 12 4 represent?
It likely refers to specific sections or chapters in a textbook or educational resource.
What are transmembrane proteins?
Integral proteins that span the membrane.
What is the functional significance of cell size?
Cell size affects the efficiency of nutrient uptake and waste removal; smaller cells have a higher surface area-to-volume ratio, facilitating these processes.
What function does the cytoskeleton provide to the cell?
It gives mechanical support to the cell.
How do Paramecium manage excess water in a hypotonic environment?
They use a contractile vacuole to pump excess water out of the cell.
What lines the inner side of the plasma membrane that forms vesicles during pinocytosis?
Coat proteins, forming coated vesicles.
What structure is composed of outer microtubule doublets and a central microtubule?
Basal body.
What is the function of contractile vacuoles?
They pump excess water out of protist cells.
What is biological size?
The measurement of organisms and cells, often expressed in micrometers or nanometers.
What are microfilaments primarily composed of?
Actin and myosin.
What is the endomembrane system?
A network of membranes that includes the nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, and vesicles.
What is the diameter of microfilaments?
7 nm.
What organelle is responsible for protein synthesis in the endomembrane system?
The endoplasmic reticulum (ER).
What field of study uses microscopes as important tools?
Cytology, the study of cell structure.
How do membrane proteins contribute to cell signaling?
By acting as receptors for signaling molecules.
What is signal transduction in the context of membrane proteins?
It involves a membrane protein binding to a chemical messenger, causing a conformational change that relays the message inside the cell.
What structure is associated with radial spokes in a basal body?
Outer doublets.
Can lipids and proteins move within the membrane?
Yes, most lipids and some proteins can move sideways.
What is the primary function of catalase in peroxisomes?
Converts hydrogen peroxide (H2O2) to water and oxygen (O2).
What characteristic do most membrane proteins have?
They are amphipathic.
Where can ribosomes be found in a cell?
Bound to the endoplasmic reticulum or free in the cytoplasm.
What is one function of membrane proteins?
Transporting substances across the cell membrane.
What are the two major types of membrane proteins?
Peripheral proteins and integral proteins.
In which journal was the study published?
Applied Microbiology and Biotechnology.
What are the main components of cellular membranes?
Lipids and proteins, with carbohydrates also being important.
What is one of the main functions of the Golgi apparatus?
Modifies products of the endoplasmic reticulum (ER).
Where are the hydrophobic regions of membrane proteins located?
Embedded in the bilayer.
What are microtubules made of?
Protein tubulin.
What occurs if the surrounding solution has a lower concentration of solutes than the inside of the cell?
Water will tend to enter the cell.
What is the extracellular matrix (ECM)?
A network of fibers and proteins outside of cells that provides structural and biochemical support.
What is the primary function of the nucleus?
Controls cellular activities.
What substance impregnates the secondary cell wall?
Lignin, forming a hard and impervious layer.
How does the length of fatty acid tails affect membrane fluidity?
Shorter fatty acid tails increase membrane fluidity.
What happens to chromatin as a cell prepares to divide?
It condenses to form discrete chromosomes.
How does the Golgi apparatus handle materials?
It sorts and packages materials into transport vesicles.
What is the wall thickness of microtubules?
5 nm.
What do the hydrophobic regions of an integral protein consist of?
Nonpolar amino acids, often coiled into α helices.
Why is the surface area to volume ratio critical for cells?
It affects the efficiency of nutrient and waste exchange.
What distinguishes prokaryotic cells from eukaryotic cells?
Prokaryotic cells lack a nucleus and membrane-bound organelles.
What is a characteristic of transmembrane proteins?
They completely span the membrane.
What role do transport proteins play in the cell membrane?
They provide a hydrophilic channel for selective solute transport or shuttle substances by changing shape.
What is the function of pili in prokaryotes?
Attachment structures on the surface of some prokaryotes.
What is required for active transport?
ATP and a carrier protein.
Give an example of ions involved in facilitated diffusion in nerve cells.
Sodium (Na) and Potassium (K) ions.
What is the typical diameter range of prokaryotic cells?
0.5 – 5 μm.
What is one major function of membrane proteins related to transport?
Facilitating the movement of substances across the cell membrane.
What type of site is involved in active transport?
A specific receptor site.
What does a cross section of a centriole reveal?
The arrangement of microtubules within the centriole.
What is the function of confocal microscopy?
Uses lasers and special optics for 'optical sectioning' of fluorescently-stained specimens.
What forms can eukaryotic cells take?
Unicellular, multicellular, or filamentous.
In which direction do free water molecules diffuse during osmosis?
From lower solute concentration to higher solute concentration.
What is the main characteristic of the Brightfield technique for unstained specimens?
It passes light directly through the specimen, resulting in little contrast unless the cell is naturally pigmented or stained.
Where are the hydrophilic regions of membrane proteins oriented?
Toward the cytosol and extracellular fluid inside and outside the membrane.
How are centrioles arranged?
In a small cylindrical shape, perpendicular to one another.
Why do organisms increase unsaturated phospholipids in colder temperatures?
To prevent membrane solidification.
What is the main electrogenic pump in plants, fungi, and bacteria?
The proton pump, which actively transports hydrogen ions (H+) out of the cell.
What is formed around the material during endocytosis?
A vesicle for transport.
What is membrane potential?
The voltage across a membrane.
How does temperature affect diffusion?
Higher temperatures increase the rate of diffusion.
Where are receptor proteins clustered in receptor-mediated endocytosis?
In coated pits.
What is the state of plant cells in an isotonic solution?
They become flaccid (limp) and the plant wilts.
What are the two types of special transport proteins involved in facilitated diffusion?
Channel proteins and carrier proteins.
What does the fluid mosaic model of membrane structure depict?
The membrane as a mosaic of protein molecules bobbing in a fluid bilayer of phospholipids.
What is passive transport?
The movement of substances down their concentration gradients across a membrane without energy expenditure by the cell.
Are proteins randomly distributed in the membrane according to the fluid mosaic model?
No, they often form groups that carry out common functions.
What is exocytosis?
A process where transport vesicles migrate to the membrane, fuse with it, and release their contents outside the cell.
What is the function of proteins that adhere to the extracellular matrix (ECM)?
They coordinate extracellular and intracellular changes.
What is the energy and carbon source for chemoautotrophs?
They obtain energy chemically and carbon from fixing CO2.
What are the proteins that connect outer doublets in a basal body?
Cross-linking proteins.
How do some transport proteins use energy?
They hydrolyze ATP to actively pump substances across the membrane.
What is the diameter of a microtubule?
0.25 μm.
What is the function of chloroplasts?
Site of photosynthesis.
How do microfilaments change their length?
They change their length very quickly depending on locations and functions.
What do leucoplasts store?
Starch, found in roots.
What is the approximate size of mitochondria?
About 100 μm.
What is a key characteristic of microfilaments?
They are dynamic.
What characterizes Rough ER?
Its surface is studded with ribosomes.
What is a limitation of standard fluorescence micrographs?
They can appear blurry, especially in relatively thick tissue.
What is the structure of the flagellum in eukaryotes?
Complex, with a '9+2' pattern of triplet microtubules.
Is a spindle present during cell division in prokaryotes?
No spindle during cell division.
What is the primary purpose of cell fractionation?
To isolate and study different organelles within a cell.
What role do cholesterol molecules play in membrane fluidity?
Cholesterol helps to stabilize membrane fluidity across temperature changes.
What are intermediate filaments?
A type of cytoskeletal filament.
What is turgor pressure?
The pressure exerted by the inelastic cell wall on a swollen plant cell.
What does the Phase-contrast technique do?
It enhances contrast in unstained cells by amplifying variations in density, making it useful for examining living, unpigmented cells.
What happens to the net movement of solute in passive transport when equilibrium is reached?
The net movement of solute stops.
What types of junctions can membrane proteins of adjacent cells form?
Gap junctions and tight junctions.
What type of structure do the polypeptides in intermediate filaments have?
Only secondary coiled structure.
What is passive transport?
Movement of substances across the plasma membrane without the use of energy.
How can cellular membranes be described?
As fluid mosaics of lipids and proteins.
What are food vacuoles formed by?
Phagocytosis.
How do photoautotrophs obtain energy and carbon?
They get energy from light and carbon from fixing CO2.
If a transport protein is required in passive transport, what is its characteristic?
It is specific.
What are the two membranes that enclose mitochondria?
A smooth outer membrane and an inner membrane folded into cristae.
What do transport vesicles from the rough ER contain?
Secretory proteins surrounded by membranes.
What is osmosis?
The diffusion of water across a selectively permeable membrane.
What are the three types of cytoskeletal fibers?
Microtubules, microfilaments, and intermediate filaments.
What is the relationship between cell size and metabolic activity?
Larger cells may have lower metabolic rates per unit volume, while smaller cells can maintain higher metabolic activity due to better nutrient exchange.
What types of molecules can diffuse through the lipid bilayer?
Hydrophobic molecules and very small uncharged polar molecules.
What is active transport?
The movement of substances against their concentration gradient, requiring energy.
What is facilitated diffusion?
The process where many hydrophilic substances diffuse through membranes with the assistance of transport proteins.
What does the plasma membrane do in a prokaryotic cell?
It encloses the cytoplasm.
What does the smooth ER store?
Calcium ions.
What is the primary energy source for active transport?
ATP.
What proteins anchor desmosomes in the cytoplasm?
Intermediate filaments made of sturdy keratin proteins.
What is the typical shape and size of Bacillus coagulans?
A typical rod-shaped bacterium, 0.5 μm.
Is a capsule present in prokaryotic cells?
May be present and made of glycoprotein.
What organelle is involved in photosynthesis in eukaryotes?
Chloroplasts.
How does a light microscope (LM) work?
Visible light is passed through a specimen and then through glass lenses.
What are membranes primarily composed of?
Phospholipids.
What role do membrane proteins play in enzymatic activity?
They can function as enzymes to catalyze reactions.
What technique is commonly used in cell fractionation?
Centrifugation.
How do microtubules assist in organelle movement?
They guide the movement of organelles.
What role does the surface area of the membrane play in diffusion?
A larger surface area increases the rate of diffusion.
What does the nucleus membrane control?
The inflow and outflow of material to and from the nucleus.
How are the microfibrils arranged in the primary cell wall?
They are randomly arranged.
What occurs to plant cells in a hypertonic environment?
They lose water, causing the cell to shrivel and the membrane to pull away from the cell wall.
What is the nucleolus?
A structure located within the nucleus that is the site of ribosomal RNA (rRNA) synthesis.
What is the role of the nucleolus?
Acts as the site of ribosome construction.
What feature is usually present in the primary cell wall for transport?
Plasmodesmata.
Why do some cells have elongated shapes?
Elongated shapes can enhance the cell's ability to cover more area for absorption or to facilitate movement.
What type of cells commonly use exocytosis?
Secretory cells.
What is the function of the cristae in mitochondria?
To increase the surface area for biochemical reactions.
What is cell diversity?
The variety of different cell types and their functions within an organism.
What is found in the mitochondrial matrix?
Free ribosomes and mitochondrial DNA.
What are the two main categories of cells?
Prokaryotic and Eukaryotic cells.
How can enzymes in a membrane be organized?
They can be organized as a team to carry out sequential steps of a metabolic pathway.
What pigment is contained in chloroplasts?
Chlorophyll.
What do chromoplasts store?
Carotenoid pigments (red, orange, and yellow in color).
What is the function of vesicles in the endomembrane system?
They transport materials between different organelles.
What is the function of membrane proteins in cell adhesion?
They help cells stick to each other and to the extracellular matrix.
What types of molecules can pass through gap junctions?
Ions, sugars, amino acids, and other small molecules.
What type of endocytosis involves receptors?
Receptor-mediated endocytosis.
Are pili present in eukaryotes?
Absent.
What is the vesicle for nitrogen fixation in prokaryotes?
Plasma membrane.
What type of macromolecules does the Golgi apparatus manufacture?
Certain macromolecules.
What is the cytoskeleton?
A network of fibers that organizes structures and activities in the cell.
What creates voltage across a membrane?
Differences in the distribution of positive and negative ions.
In what way do membrane proteins assist in cell recognition?
By serving as markers for cell identification.
What is formed when a cell engulfs a particle during phagocytosis?
A membranous sac called a food vacuole.
What are the two combined forces that drive the diffusion of ions across a membrane?
The chemical force (ion's concentration gradient) and the electrical force (membrane potential effect).
What is a key function of the rough endoplasmic reticulum (Rough ER)?
It has bound ribosomes that secrete glycoproteins.
How do microtubules contribute to cytoplasmic organization?
They divide the cytoplasm into compartments, allowing specialized enzymes to function better.
What is the phenomenon called when the cell membrane pulls away from the cell wall?
Plasmolysis.
What is a key characteristic of eukaryotic cells?
They have a nucleus and membrane-bound organelles.
How do the sizes of eukaryotic cells compare to prokaryotic cells?
Eukaryotic cells are generally much larger than prokaryotic cells.
Where are central vacuoles found?
In plant cells.
Where is the DNA located in a prokaryotic cell?
In the nucleoid region, which is not enclosed by a membrane.
What do central vacuoles hold?
Reserves of important organic compounds and water.
What is the relationship between the ER membrane and the nuclear envelope?
The ER membrane is continuous with the nuclear envelope.
How does the smooth ER contribute to detoxification?
It detoxifies drugs and poisons.
What is the diameter of the central microtubule in a basal body?
0.1 μm.
What is a characteristic of eukaryotic cells?
They have a nucleus and membrane-bound organelles.
How do membrane proteins assist in enzymatic activity?
By acting as enzymes that catalyze biochemical reactions.
What is a granum?
A stack of thylakoids.
How many grana can each chloroplast contain?
40 to 60 grana.
What is the structure of the flagellum in prokaryotes?
Fine, simple & only consists of one microtubule.
Does passive transport always require a transport protein?
It may or may not need a transport protein.
How do microfilaments contribute to cell structure?
They bond to membrane proteins, helping maintain cell shape and stabilize protein location.
Which fields help correlate cell function with structure?
Biochemistry and cytology.
How does a scanning electron microscope (SEM) work?
It focuses a beam of electrons onto the surface of a specimen.
Where is the cytoplasm located in eukaryotic cells?
In the region between the plasma membrane and the nucleus.
How can the rate of diffusion be increased?
By transport proteins in the membrane.
What is achieved when the concentration of molecules is equal on both sides of the membrane?
Equilibrium.
What are centrioles?
Cylindrical structures found in the centrosome, involved in cell division.
What is the main purpose of differential interference contrast microscopy?
To exaggerate differences in density, making the image appear almost 3D.
What percentage of the total membrane does the endoplasmic reticulum (ER) account for in many eukaryotic cells?
More than half.
What is the function of a membrane protein that acts as an enzyme?
It may have its active site exposed to substances in the adjacent solution, facilitating enzymatic activity.
What is the primary function of chloroplasts?
Capture of light energy.
What role does the Golgi apparatus play in the endomembrane system?
It modifies, sorts, and packages proteins and lipids for secretion or delivery to other organelles.
What are the two types of transport proteins involved in facilitated diffusion?
Channel proteins and carrier proteins.
What is the function of the cell wall in prokaryotes?
It is a rigid structure outside the plasma membrane.
Why is cell diversity important?
It allows for specialization and adaptation to different functions and environments.
What is the arrangement of microtubules in a basal body?
Triplet arrangement.
What is pinocytosis?
Cellular drinking.
What is the structure of genetic material in prokaryotes?
DNA is circular, lies free in the cytoplasm, and is naked.
What type of ribosomes do prokaryotes have?
Smaller types, 70 S (18 nm).
How does cell shape influence its function?
Cell shape can determine the cell's ability to perform specific functions, such as movement, communication, and interaction with other cells.
What structures are made up of protein tubulin?
Cilia, flagella, and spindle fibers.
What type of environment do Paramecium live in?
A hypotonic environment.
What happens to the surface area and volume of a cell as it increases in size?
Volume grows proportionately more than surface area.
Where do integral proteins often interact?
With the extracellular side of the membrane.
How does the distance over which diffusion occurs affect the process?
Longer distances decrease the rate of diffusion.
What is facilitated diffusion?
The process of transporting substances across a membrane with the help of carrier proteins.
What is one of the main functions of the smooth endoplasmic reticulum (Smooth ER)?
It synthesizes lipids.
What is the role of ribosomes in prokaryotic cells?
They synthesize proteins.
What is the typical diameter range of eukaryotic cells?
10 – 100 μm.
What allows microfilaments to contract?
Subunits of proteins that can slide over one another.
What role do membrane proteins play in cell recognition?
They serve as markers for cell identification.
What is the result of using confocal microscopy?
A sharp image due to the subtraction of out-of-focus fluorescence.
What is the function of flagella in bacteria?
They are locomotion organelles.
How many organelles do prokaryotic cells typically have?
Few organelles, none are membrane-bound.
In which tissues are gap junctions particularly important?
Heart muscle and animal embryos.
Is a capsule present in eukaryotic cells?
Absent.
Is there a vesicle for nitrogen fixation in eukaryotes?
None.
What is the first principle of Cell Theory?
All living things are composed of cells.
What happens to emptied receptors after endocytosis?
They are recycled to the plasma membrane by the same vesicle.
What does the negative charge inside the cell favor?
Passive transport of cations into and anions out of the cell.
What do chromosomes carry?
Hereditary information.
What can happen to a plant if it experiences plasmolysis?
The plant will wilt and may die.
What is the significance of the cell membrane?
It regulates the movement of substances in and out of the cell.
What is an example of a product secreted by exocytosis?
Insulin, secreted by cells in the pancreas.
How does fluorescence microscopy show the locations of specific molecules in a cell?
By tagging the molecules with fluorescent dyes or antibodies.
What type of section shows the structure of a centriole?
Longitudinal section.
What type of radiation do fluorescent substances absorb?
Ultraviolet radiation.
How does cryo-EM aid in the search for a COVID-19 vaccine?
By providing insight into the spike-protein structure.
What characterizes Smooth ER?
It lacks ribosomes.
What is the capsule in prokaryotic cells?
A jelly-like outer coating of many prokaryotes.
What do gap junctions provide?
Cytoplasmic channels from one cell to an adjacent cell.
Why are gap junctions important?
They are necessary for communication between cells in many types of tissues.
How many types of endocytosis are there?
Three types.
What type of ribosomes do eukaryotes have?
Bigger types, 80 S (22 nm).
What does an ion do when it diffuses down its electrochemical gradient?
It moves from an area of higher concentration to an area of lower concentration, influenced by electrical forces.
What is active transport?
The movement of substances across a membrane against their concentration gradients using energy, usually supplied by ATP.
What is the structure of spindle fibers?
They consist of 9 triplets of microtubules.
How does cryo-EM complement X-ray crystallography?
It reveals protein complexes and subcellular structures.
What do chemoheterotrophs use for energy and carbon?
They obtain both energy and carbon from organic compounds by ingesting organisms or materials with carbon.
What do tight junctions prevent?
Fluid from moving across a layer of cells.
What is a characteristic of prokaryotic cells?
They lack a nucleus and membrane-bound organelles.
What forms can prokaryotic cells take?
Unicellular or filamentous.
What is the function of desmosomes?
To fasten cells together into strong sheets.
What is the composition of the cell wall in prokaryotes?
Peptidoglycan.
How many thylakoids can each granum contain?
10 to 100 thylakoids.
How many organelles do eukaryotic cells typically have?
Many organelles, some are membrane-bound.
What organelle is involved in respiration in prokaryotes?
Plasma membrane.
What are the two distinct regions of the endoplasmic reticulum?
Smooth ER and Rough ER.
How do lysosomes function within the endomembrane system?
They contain enzymes that break down waste materials and cellular debris.
What is the role of a receptor protein in signal transduction?
It has a binding site that fits the shape of a specific chemical messenger, such as a hormone.
In confocal microscopy, what colors represent nerve cells and support cells?
Nerve cells are green, support cells are red, and regions of overlap are yellow.
What organelle is involved in respiration in eukaryotes?
Mitochondria.
What is the basic unit of all organisms according to Cell Theory?
Cells.
What is a prokaryotic cell?
A cell without a true nucleus or other internal membranes.
What surrounds the microtubules in a basal body?
Plasma membrane.
What materials make up the cell wall in eukaryotes?
Cellulose and chitin.
What is the structure of genetic material in eukaryotes?
DNA is linear and contained in the nucleus.
Is a spindle present during cell division in eukaryotes?
Spindle present during cell division.
What is the stroma in chloroplasts?
The internal fluid of the chloroplast.
How do membrane proteins contribute to the structural integrity of the cell?
By providing a framework that supports the cell's shape.
What is the function of pili in prokaryotes?
May be present for attachment.
What organelle is involved in photosynthesis in prokaryotes?
Folding of plasma membrane.
What is a eukaryotic cell?
A cell with a true nucleus and organelles surrounded by a membrane.
What distinguishes prokaryotic cells from eukaryotic cells?
Prokaryotic cells lack a true nucleus and membrane-bound organelles, while eukaryotic cells have both.
What connects the nuclear envelope to the endoplasmic reticulum?
The outer membrane of the nuclear envelope is continuous with the endoplasmic reticulum.
What is phagocytosis?
Cellular eating.
What are lamellae in chloroplasts?
Channels that connect granum.